Articles in the Category ”Products”

Generate and purify Fab fragments from human and mouse IgG using Genovis SmartEnzymes

Antibodies are important tools in several scientific research areas, and sometimes it is necessary to cleave antibodies to generate antigen binding (Fab) fragments. The Fab fragments can be used within e.g. imaging, removal of effector functions, infection biology, binding studies, and mass spectrometry.

In a recently published book (“Bacterial Pathogenesis, Methods and Protocols”, Humana Press, 2017), the Genovis Team has written a chapter of how to generate and purify Fab fragments from human and mouse IgG by using the bacterial proteases IdeS (FabRICATOR), SpeB (FabULOUS) and Kgp (GingisKHAN).

 

  • The FabRICATOR enzyme is derived from Streptococcus pyogenes, and generates F(ab’)2 fragments in all human IgG subclasses. A homogenous pool of Fab’ fragments can be generated under mild reducing conditions. Read more about FabRICATOR here.
  • The FabULOUS enzyme is also derived from Streptococcus pyogenes, and can be used to digest mouse IgG. Using light chain affinity resins, Fab fragments can be purified. Read more about FabULOUS here.
  • The GingisKHAN enzyme is derived from Porphyromonas gingivalis, digests the upper hinge and generates intact Fab fragments from human IgG1 in one step. Using a CH1-specific affinity resin, the Fab fragments can be purified. Read more about GingisKHAN here.

Pathogenesis Book
Find the full text paper here:
Sjögren, J. et al., 2017. Generating and Purifying Fab Fragments from Human and Mouse IgG Using the Bacterial Enzymes IdeS, SpeB and Kgp. Methods in Molecular Biology, 1535, pp.319–329.

FabULOUS Fab Kit – Intact Fab fragments from mouse IgG

June 7, 2016 | Products |

FabFabKit

The FabULOUS (SpeB) enzyme digests IgG in the hinge region. The FabULOUS Fab kit is designed for digestion and purification of Fab fragments from mouse IgG antibodies. Incubation at optimized reducing conditions generates intact Fab, easily purified with spin columns.

  • Digestion in the hinge with FabULOUS
  • Affinity purification of Fab fragments using the light chain
  • Preparation of Fab fragments from mouse IgG in less than 2 h

Read more about FabULOUS Fab kit and order now

GingisREX – New Enzyme from Genovis

GingisRex_Logo_Gubbe

Genovis expands the protease portfolio with GingisREX – a new enzyme with unique specificity for arginine residues. The GingisREX protease can be used for peptide mapping, protein sequencing and post-translational modification analysis using mass spectrometry.

Press release

Read more about GingisREX

Now Available – FragIT™Z & FragIT™Z Kit

May 28, 2015 | Products |

We like to introduce FragIT Z – the immobilized version of FabRICATOR Z. Digest your mouse IgG2a more rapidly without having to remove the enzyme from the final preparation.

For separation of the generated antibody domains we also offer the convenient FragIT Z Kit which enables rapid isolation of F(ab’)2 and Fc.

Read more about these products here

Introducing FabRICATOR Z for mouse IgG2a

May 5, 2015 | Products |

We are proud to present a new member of the SmartEnzyme family: FabRICATOR Z (IdeZ).

FabRICATOR Z (IdeZ) is a cystein protease highly similar to FabRICATOR (IdeS). However, FabRCATOR Z is much more efficient in digesting mouse IgG2a and IgG3 than FabRICATOR. The new enzyme is as easy to use as “regular” FabRICATOR – add the enzyme to your mouse IgG and incubate 2 hours to get a homogenous pool of F(ab’)2 and Fc-fragments.

Read more about FabRICATOR Z here