Blog

Welcome to our blog! This is where we share a bit more – insights, updates, and thoughts from Genovis. Take a look around and see what you find.

SmartEnzymes™ in Antibody Subunit Analysis by Janssen and Celgene
2020-01-23
Scientists at Janssen and Celgene use SmartEnzymes to analyze symmetric and asymmetric structure–function relationships of modified bispecific antibodies. Forced degradation studies are useful for quickly evaluating critical quality attributes. Such modifications may have different biological impacts depending on if they occur symmetrically, in both chains of the antibody, or asymmetrically, in only one chain. In the recently published article, Evans et…
Fluorescent Imaging using GlyCLICK®, an Interview with ImaGene-iT
2020-01-14
Bo Holmqvist at ImaGene-iT gives us insight about microscopy imaging and how ImaGene-iT is using the site-specific GlyCLICK® technology to obtain high-quality fluorescence images. Tell us about yourself and ImaGene-iT? I am CSO at ImaGene-iT, an independent contract research company supporting the life science industry and academia. Concurrently, as associate professor in experimental pathology, I have a research background in histology and…
FabRICATOR®-HPLC for Automated Antibody Subunit Analysis
2019-12-17
Middle-level analysis is a universally accepted analytical strategy for rapid characterization of antibodies. The FabRICATOR® (IdeS)enzyme specifically digests IgG below the hinge, generating fragments that are suitable for high-resolution mass spectrometry. By immobilizing the FabRICATOR enzyme in an HPLC column format, a fully automated on-column digestion of IgG is possible. In a recent article…
SmartEnzymes™ Simplify Characterization of Charge Variants by Native Mass Spectrometry
2019-11-19
Monoclonal antibodies (mAbs) are complex macromolecules that undergo a wide range of post-translational modifications that can result in charge heterogeneity. Ion exchange (IEX) chromatography is considered the gold standard for characterization of charge variants. This approach separates charge variants and allows for the collection of isoforms for protein identification by mass spectrometry (MS). However, the…
Monitoring Glycation Levels on Bispecific Biologics using FabRICATOR®
2019-11-18
Bispecific monoclonal antibodies (BsAbs) are multi-functioning and complex biologics with the ability to recognize two different epitopes for improved therapeutic properties. Characterizing protein modifications such as glycation on biologics is vital to ensure consistency in stability and function. The structural complexity of BsAbs requires robust analytical methods, where conventional top-down and bottom-up strategies may lack…
SmartEnzymes™ in a new approach to characterize ADCs
2019-10-25
Antibody drug conjugates (ADCs) consist of monoclonal antibodies chemically linked to a cytotoxic agent. The target specificity of the monoclonal antibody in combination with the potency of the cytotoxic drug make ADCs promising therapeutic agents. However, the molecules are often complex, making evaluation of the quality attributes for the ADC challenging. In order to…
Characterizing ADCs using FabRICATOR and middle-down MS
2019-09-24
The process of characterizing an antibody drug conjugate (ADC) requires the evaluation of critical quality attributes including primary sequence analysis and drug conjugation assessment. Addressing glycoprofile determination as well as drug load distribution and drug-to-antibody ratio (DAR) is however challenging using peptide mapping. In addition, further challenges arise from the increased hydrophobicity of the ADC…
SmartEnzymes at BGI, an interview with Aaron Bailey
2019-09-24
We got the opportunity to talk to Aaron Bailey at BGI Americas. From their newly setup MS labs, they use SmartEnzymes to help provide analytical services to their customers globally. Read on to find out more. Tell us about yourself and BGI? Currently I work at BGI Americas as the Associate Director of Product Management for Mass Spectrometry…
Introducing our new CFO, Johny Humaloja
2019-09-09
Johny joined the Genovis team in August, and he brings more than 25 years of financial experience to the company. We are very excited to have you in the team, Johny! Welcome! Tell us a bit about yourself I live in Malmö, in the southern part of Sweden and I have two daughters. I…
FragIT™ kit in Study Evaluating IgG Charge
2019-09-02
Protein charge is a fundamental property that influences both the ability to interact with other molecules and the structure, solubility and stability of the protein. In this collaborative work by Boehringer Ingelheim Pharmaceuticals, Janssen BioTherapeutics and University of New Hampshire, the charge measurements of twelve monoclonal IgG and their F(ab’)2 and Fc fragments are…
SmartEnzymes™ in the Manufacturing Process for a Biological Drug
2019-08-20
We have received an order for SmartEnzymes that will be used in the manufacturing process for a biological drug.
Improved antibody-PET tracers for in vivo imaging with GlyCLICK®
2019-07-08
Radioactively labelled antibodies are excellent immuno-PET tracers for evaluating in vivo distribution and performance of therapuetic agents. Site-specific conjugation at the antibody Fc glycan site by enzymatic remodeling allows for a uniform label distribution of such PET-tracers, compared to conjugates generated with conventional random labelling strategies. In an article by Kristensen et al. (2019), the…