LC-MS Analysis Service of Recombinant Antibodies
We have entered a service agreement with evitria AG (Zürich, Switzerland) to offer a fast and high-quality LC-MS characterization of recombinant antibodies using our SmartEnzymes™ workflows!
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Welcome to our blog! This is where we share a bit more – insights, updates, and thoughts from Genovis. Take a look around and see what you find.
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NEW! Hydrolysis of Flexible Linkers using GlySERIAS
GlySERIAS is a unique enzyme that digests flexible glycine-rich fusion protein linkers such as Gly4Ser and GlyxSery (GS) and polyglycine (G) linkers. The enzyme enables separation of the individual domains of multi-functional fusion proteins to facilitate characterization and increase the understanding of these molecules. Middle-level analysis of fusion proteins serves to both reduce the…
NEW! Sugar-free Proteins in a Few Hours using OmniGLYZOR
OmniGLYZOR hydrolyzes N- and mucin-type O-glycans. It contains a mixture of immobilized enzymes for fast and efficient removal of N- and simple mucin-type O-glycans on antibodies, fusion proteins and other glycosylated proteins. Removal of glycans is widely used to reduce heterogeneity to facilitate analysis of the protein by for example mass spectrometry. Deglycosylation can also…
SmartEnzymes™ for Gene Therapy
The development of advanced gene therapies is promising and gives thousands of patients new hope for a curative treatment. However, many of the current gene therapies are using adeno-associated viruses as transporters of new genetic material. Up to 60% of patients may have antibodies against this viral vehicle and this is usually an exclusion criteria…
NEW! Azide Activation of Human IgG using TransGLYCIT Azide Activation
We're happy and proud to announce the launch of TransGLYCIT Azide Activation!
NEW! PNGase F for Hydrolysis of N-glycans
We are happy to announce that PNGase F for in-solution hydrolysis of N-glycans from glycoproteins is added to our portfolio of SmartEnzymes!
NEW FabDELLO – The SmartEnzyme for Mutated IgG
We introduce FabDELLO, a protease that specifically digests human IgG1 at a single site above the hinge, generating intact Fab and Fc fragments within two hours with no need for reducing conditions. The enzyme is active on antibodies with mutated hinge regions and enables middle-level LC-MS for characterization of critical quality attributes.
NEW SmartEnzyme – Immobilized PNGase F for Fast N-glycan Hydrolysis!
Genovis launches Immobilized PNGase F, a resin with the PNGase F enzyme covalently coupled to agarose beads for hydrolysis of N-glycans on antibodies, fusion proteins and other N-glycosylated proteins in a convenient spin column format.
NEW TransGLYCIT™ Glycoforms Available!
Genovis launches new glycoforms with TransGLYCIT™ - A platform to create IgG with defined glycan profiles! TransGLYCIT enables specific IgG N-glycan remodeling and prepares antibodies with a defined and homogenous G0, G1, G2 or G2S2 glycoform using fast and robust enzymatic workflows. With the optional FucosEXO™ 16 enzyme, afucosylated antibodies can be obtained that enable…
New Application Note on Antibody Oxidation Analysis
A Rapid LC-MS Assay for Monitoring of mAb Oxidation at the Subunit Level Methionine oxidation is considered a critical quality attribute of therapeutic antibodies and may impact the clinical safety and efficacy. Therefore, monitoring of methionine oxidations is required during the discovery, development, and production of therapeutic antibodies. Traditional methods to characterize oxidation rely…
Launching New Anti-FabRICATOR Formats!
We are happy to announce two new affinity purified antibody formats for detecting the FabRICATOR enzyme! The Anti-FabRICATOR Affinity Purified is an affinity purified goat polyclonal antibody and the Anti-FabRICATOR Affinity Purified Biotin Conjugated is an affinity purified and biotin-conjugated goat polyclonal antibody.
Characterization of SARS-CoV-2 Receptor-Binding Domains using SmartEnzymes™
Scientists at Leiden University Medical Center (LUMC) present a multilevel mass spectrometry approach using SmartEnzymes for in-depth characterization of mammalian SARS-CoV-2 receptor-binding (RBD) domains. The COVID-19 disease caused by the SARS-CoV-2 virus has affected more that 100 million individuals in the ongoing pandemic. The enveloped RNA corona virus contains three structural proteins in the…
