Frequently Asked Questions
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What antibody species can I transglycosylate using TransGLYCIT?
The TransGLYCIT platform is developed for transglycosylation of human IgG.
Learn more about TransGLYCIT®
What is the substrate specificity of GlycINATOR?
GlycINATOR is an IgG specific endoglycosidase that hydrolyzes complex, hybrid and high mannonse type glycans on the conserved Fc site on IgG.
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Does TransGLYCIT work on any glycoprotein?
No, TransGLYCIT is based on IgG specific enzymes and will only transglycosylate IgG.
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Which buffers can be used with the PNGaseF Immobilized column?
The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral pH containing 0.15 M NaCl can also be used but optimization may then be required.
Learn more about PNGase F
Can the deglycosylation on the column be performed under denaturing conditions?
The columns are recommended to be used for deglycosylation at native conditions only.
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Is it possible the reuse the PNGAse F Immobilized column?
No, it is not recommended. We can only guarantee optimal performance for one-time use.
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Does PNGase F Immobilized remove all N-linked glycans?
PNGase F Immobilized hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.
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Is it possible to purchase PNGaseF Immobilized in bulk?
Yes! Contact us at info@genovis.com
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Can OmniGLYZOR be used for released glycan analysis?
No, both N- and O-glycans are trimmed by the exoglycosidases present in OmniGLYZOR and do not longer represent the structures found on the intact glycoprotein substrate.
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Does OmniGLYZOR remove all N-glycans?
OmniGLYZOR hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.
Learn more about OmniGLYZOR®