Frequently Asked Questions
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Does TransGLYCIT work on all human IgG subclasses?
Yes, the transglycosylation reaction can be performed on all subclasses of human IgG. The reaction is somewhat slower on IgG2 and longer incubation times may be necessary to obtain over 95% transglycosylation.
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Can I use the FucosEXO 16 enzyme on native antibodies to remove the core fucose?
Unfortunately no, the enzyme requires trimming of the Fc-glycan using GlycINATOR to enable access to the core fucose substrate.
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What antibody species can I transglycosylate using TransGLYCIT?
The TransGLYCIT platform is developed for transglycosylation of human IgG.
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What is the substrate specificity of GlycINATOR?
GlycINATOR is an IgG specific endoglycosidase that hydrolyzes complex, hybrid and high mannonse type glycans on the conserved Fc site on IgG.
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Does TransGLYCIT work on any glycoprotein?
No, TransGLYCIT is based on IgG specific enzymes and will only transglycosylate IgG.
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Which buffers can be used with the PNGaseF Immobilized column?
The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral pH containing 0.15 M NaCl can also be used but optimization may then be required.
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Can the deglycosylation on the column be performed under denaturing conditions?
The columns are recommended to be used for deglycosylation at native conditions only.
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Is it possible the reuse the PNGAse F Immobilized column?
No, it is not recommended. We can only guarantee optimal performance for one-time use.
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Does PNGase F Immobilized remove all N-linked glycans?
PNGase F Immobilized hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.
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Is it possible to purchase PNGaseF Immobilized in bulk?
Yes! Contact us at info@genovis.com
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