When and why should I use the OpeRATOR elution procedure?

The OpeRATOR enzyme digests O-glycosylated proteins N-terminally of the serine (S) and threonine (T) glycosylation sites. If you want to know at which S or T a specific O-glycan is sited, we recommend elution with OpeRATOR. We also recommend elution with OpeRATOR if you have a glycoprotein with suitable distances between the O-glycan sites and suitable distances between the O-glycans and the N/C-terminals, and you want to generate peptides of suitable size for your purpose.