Question 1

What is the difference between FabALACTICA and GingisKHAN?

Felicia Mejare · Accepted Answer

FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (...KSCDKT / HTCPPCP...) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (...KSCDK/THTCPPCP...).

Question 2

What is the difference between FabALACTICA and GingisKHAN?

Felicia Mejare · Accepted Answer

FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (...KSCDKT / HTCPPCP...) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (...KSCDK/THTCPPCP...).

Question 3

What is the difference between FabALACTICA and GingisKHAN?

Felicia Mejare · Accepted Answer

FabALACTICA (IgdE) and GingisKHAN (Kgp) are both cysteine proteases that digest IgG1 above the hinge to generate intact Fab and Fc fragments. FabALACTICA is specific for human IgG1 and digests at a specific site (...KSCDKT / HTCPPCP...) with overnight incubation without the need for reducing conditions. GingisKHAN is a general protease that with the recommended reaction conditions, 1 hour with light reducing conditions, digests human IgG1 at a specific site (...KSCDK/THTCPPCP...).