Question 1

Can I use GingisKHAN on other subclasses than human IgG1?

Felicia Mejare · Accepted Answer

No. GingisKHAN is a protease that digests at exposed Lysines with no secondary structure restrictions. At the recommended reaction conditions, GingisKHAN digestion above the hinge has only been confirmed on human IgG1 with the exposed lysine above the hinge, (...KSCDK / THTCPPCP...). No digestion has been confirmed on other subclasses of human IgG except for several digestion sites in human IgG3.

Question 2

Can I use GingisKHAN on other subclasses than human IgG1?

Felicia Mejare · Accepted Answer

No. GingisKHAN is a protease that digests at exposed Lysines with no secondary structure restrictions. At the recommended reaction conditions, GingisKHAN digestion above the hinge has only been confirmed on human IgG1 with the exposed lysine above the hinge, (...KSCDK / THTCPPCP...). No digestion has been confirmed on other subclasses of human IgG except for several digestion sites in human IgG3.

Question 3

Can I use GingisKHAN on other subclasses than human IgG1?

Felicia Mejare · Accepted Answer

No. GingisKHAN is a protease that digests at exposed Lysines with no secondary structure restrictions. At the recommended reaction conditions, GingisKHAN digestion above the hinge has only been confirmed on human IgG1 with the exposed lysine above the hinge, (...KSCDK / THTCPPCP...). No digestion has been confirmed on other subclasses of human IgG except for several digestion sites in human IgG3.