Question 1

Can I digest Fc-fusion proteins using GingisKHAN (Kgp)?

Felicia Mejare · Accepted Answer

Yes, GingisKHAN should be able to digest a Fc fusion protein if you have not fused the protein too close to the cleavage site and the Fc-part is from a human IgG1. GingisKHAN digests human IgG1 just above the hinge (...KSCDK / THTCPPCP...). If there are exposed Lysines in the fusion part, digestion might occur there as well.

Question 2

Can I digest Fc-fusion proteins using GingisKHAN (Kgp)?

Felicia Mejare · Accepted Answer

Yes, GingisKHAN should be able to digest a Fc fusion protein if you have not fused the protein too close to the cleavage site and the Fc-part is from a human IgG1. GingisKHAN digests human IgG1 just above the hinge (...KSCDK / THTCPPCP...). If there are exposed Lysines in the fusion part, digestion might occur there as well.

Question 3

Can I digest Fc-fusion proteins using GingisKHAN (Kgp)?

Felicia Mejare · Accepted Answer

Yes, GingisKHAN should be able to digest a Fc fusion protein if you have not fused the protein too close to the cleavage site and the Fc-part is from a human IgG1. GingisKHAN digests human IgG1 just above the hinge (...KSCDK / THTCPPCP...). If there are exposed Lysines in the fusion part, digestion might occur there as well.