Is it possible to purchase Immobilized PNGaseF in bulk?

Yes! Contact us at info@genovis.com

Does Immobilized PNGase F remove all N-linked glycans?

Immobilized PNGAse F hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.

Is it possible the reuse the Immobilized PNGAse F column?

No, it is not recommended. We can only guarantee optimal performance for one-time use.

Can the denaturing protocol be performed at a lower temperature than 50 °C?

Yes, it is possible to perform the incubation at 37 °C if the incubation time is prolonged. Treat the protein sample mix containing 0.5% RapiGest at 90 °C, 5 min prior to the incubation with Immobilized PNGase F.

Can the deglycosylation on the column be performed under denaturing and reducing conditions?

The columns can be used for deglycosylation at native or denaturing conditions. Reducing conditions can be used with the denaturing protocol but the reaction parameters need to be optimized for the specific substrate.

Which buffers can be used with the Immobilized PNGaseF column?

The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral pH containing 0.15 M NaCl can also be used but optimization may then be required.

Does TransGLYCIT work on any glycoprotein?

No, TransGLYCIT is based on IgG specific enzymes and will only transglycosylate IgG.

What is the substrate specificity of GlycINATOR?

GlycINATOR is an IgG specific endoglycosidase that hydrolyzes complex, hybrid and high mannonse type glycans on the conserved Fc site on IgG.

What antibody species can I transglycosylate using TransGLYCIT?

The TransGLYCIT platform is developed for transglycosylation of human IgG.

Can I use the FucosEXO 16 enzyme on native antibodies to remove the core fucose?

Unfortunately no, the enzyme requires trimming of the Fc-glycan using GlycINATOR to enable access to the core fucose substrate.

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