Can OglyZOR be used to deglycosylate peptides generated with OpeRATOR?
Yes, this workflow enables MS/MS of O-glycosylated peptides with removed O-glycans.
Will OglyZOR work on extended core 1 structures or single HexNAc?
No, OglyZOR recognizes the core 1 disaccharide HexNAcHex (or GalNAcGal) and to some extend also the core 3 disaccharide HexNAc(2) (or GalNAcGlcNAc).
Will OglyZOR leave the N-glycans intact?
OglyZOR only works on desialylated O-glycans therefore is a sialidase (SialEXO) included with the product. SialEXO is not O-glycan specific and will therefore desialylate any N-glycans that are present.
Can I use OglyZOR and PNGaseF in the same reaction?
Yes, we have performed successful experiments with OglyZOR, SialEXO and PNGaseF in the same reaction under native conditions O/N at pH ~7.0. If denaturing reaction conditions are required for PNGaseF, the reaction with OglyZOR and SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.