Can the IgASAP Sub1 digestion be performed at lower temperatures?
The optimal activity is at 37°C, but the digestion can also be performed at room temperature using a prolonged incubation time.
Which buffers are compatible with IgASAP Sub1?
The enzyme is active at pH 6.5 to 8.0, tolerates up to 300 mM NaCl, and does not require reducing conditions or co-factors for activity. PBS and TBS buffers are both compatible. Optimization may be required if buffers other than the recommended are used.
Does IgASAP Sub1 digest human IgA1-only?
IgASAP Sub1 may digest IgA1 from other primate species with similar hinge sequences, but this has not been tested. IgASAP Sub1 does not digest IgA2.