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SmartEnzymes
- - - Antibody Digestion
    - FabRICATOR^® (IdeS)
      Below hinge digestion of IgG
    - FabALACTICA^® (IgdE)
      Above hinge digestion of human IgG1
    - FabDELLO^™
      Above hinge digestion of human IgG1
    - FabRICATOR^® Z (IdeZ)
      Below hinge digestion of mouse IgG
    - GingisKHAN^® (Kgp)
      Above hinge digestion of human IgG1
    - FabULOUS^™ (SpeB)
      Above hinge digestion of IgG
    - IgASAP^™
      Digestion of IgA
    - IgMBRAZOR^™
      Digestion of IgM
  - - Fusion Protein Digestion
    - GlySERIAS^™
      Hydrolysis of Flexible Linkers
    - Antibody Conjugation
    - GlyCLICK^®
      Site-specific conjugation of IgG
    - TransGLYCIT^™
      Transglycosylation of IgG
    - Proteomics
    - GingisREX^® (RgpB)
      Arginine-specific protein digestion
  - - Glycan Profiling
    - OmniGLYZOR^™
      Hydrolysis of N- and mucin-type O-glycans
    - PNGase F
      Hydrolysis of N-glycans
    - OpeRATOR^®
      O-glycan-specific protein digestion
    - ImpaRATOR^™
      O-glycan-specific protein digestion
    - OglyZOR^®
      Hydrolysis of core 1 O-glycans
    - SialEXO^®
      Hydrolysis of sialic acids
    - FucosEXO^™
      Hydrolysis of α1-2,3,4 fucose
    - GalactEXO^™
      Hydrolysis of β1-3,4 galactose
    - GalNAcEXO^™
      Hydrolysis of α-linked GalNAcs
    - GlycOCATCH^®
      Enrichment of O-glycopeptides
  - - Antibody Deglycosylation
    - GlycINATOR^® (EndoS2)
      Hydrolysis of all Fc N-glycans
    - IgGZERO^® (EndoS)
      Hydrolysis of Fc N-glycans
    - Research Antibodies
    - Anti-FabRICATOR^®
      Detection of the FabRICATOR^® enzyme
    - Anti-FabRICATOR^® Z
      Detection of the FabRICATOR^® Z enzyme
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Genovis / FAQs / Antibody Digestion / FabRICATOR Z / FabRICATOR Z Lyophilized

Can I digest rat IgG2a with FabRICATOR Z?

Unfortunately, FabRICATOR Z does not digest rat IgG2a. If generating Fab fragments is an option, we suggest using FabULOUS enzyme that digests rat IgG2a. FabULOUS does not have a single specific cleavage site, but a primary digestion site, so there can be more than one digestion site. The digestion site(s) for rat IgG2a using FabULOUS is not determined, but the generated fragments are Fab and Fc.

Can I digest all mouse IgG subclasses with FabRICATOR Z?

No, FabRICATOR Z only digests mouse IgG2a (….CPAPNLLG / GPSVF.. ) and IgG3 (…CPAPNILG / GPSVF…) at one specific site below the hinge. If Fab fragments are an option, we recommend our FabULOUS enzyme that digest mouse IgG1 above the hinge. FabULOUS requires light reducing conditions for optimal activity. If strong reducing conditions are used, this may likely cause reduction of the interchain disulfides.