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Lyophilized enzyme for below hinge digestion of IgG. FabRICATOR (IdeS) is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.

FabRICATOR Lyophilized is available as a lyophilized powder in 2000, 5000 and 5 x 5000 (value pack) unit vials for digestion of 2, 5 and 5 x 5 mg IgG respectively, and in 8 x 100 unit strip wells and 96 x 100 unit well plates for digestion of 8 x 100 µg and 96 x 100 µg IgG respectively.
 

  • Flexible format for method development
  • Possible to combine enzymes for improved efficiency
  • Amenable to automated workflows
  • Ready to use – just add water!

Available Products

FabRICATOR Lyophilized 2000 unitsLyophilized enzyme for below hinge digestion of 2 mg IgGA0-FR1-020607.00Buy / Request a Quote
FabRICATOR Lyophilized 5000 unitsLyophilized enzyme for below hinge digestion of 5 mg IgGA0-FR1-050975.00Buy / Request a Quote
FabRICATOR Lyophilized 5 x 5000 unitsLyophilized enzyme for below hinge digestion of 5 x 5 mg IgGA0-FR1-2503,599.00Buy / Request a Quote
FabRICATOR Lyophilized 8 x 100 unitsLyophilized enzyme for below hinge digestion of 8 x 100 𝜇g IgGA0-FR1-008409.00Buy / Request a Quote
FabRICATOR Lyophilized 96 x 100 unitsLyophilized enzyme for below hinge digestion of 96 x 100 𝜇g IgGA0-FR1-0962,221.00Buy / Request a Quote

For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

FabRICATOR Lyophilized Citations

Unit Definition

One unit digests ≥ 95% of 1µg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl pH 7.4 at 37°C for 30 min.

Content and Storage

FabRICATOR Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, the enzyme is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.

Buffers

The buffers below have been tested for compatibility with FabRICATOR digestion at different pH. Note that the FabRICATOR enzyme is irreversibly inactivated below pH 5.0. Digestion above pH 8.0 requires prolonged/optimized conditions.

  • Phosphate buffer saline (PBS) 6.0 – 8.0
  • Tris buffer 7.0 – 8.0
  • MES buffer 5.5 – 6.5
  • HEPES buffer 7.0 – 8.0
  • Ammonium bicarbonate buffer 6.0 – 7.0
  • Sodium acetate buffer 6.0

FAQ and Support

Popular FAQ

No. According to the following reference, FabRICATOR does not digest IgM. Pavel-Rammingen et al., 2002. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G.

Yes. FabRICATOR is compatible with EDTA. Make sure your pH is within 6-8.

We have no stability data for storage at -20°C or -70°C. However, we have customer reporting that they successfully stored reconstituted FabRICATOR at -20°C or -70°C for a couple of months. Genovis cannot guarantee the activity of the enzyme, but these storage conditions might be OK depending on the intended application.

If we assume that the antibody concentration in serum is 15 mg/ml you would have to add 10-15 000 units to a sample of 1 ml serum. In 50 µl serum you would have to add 500 – 750 units of FabRICATOR.

Yes, PNGaseF and FabRICATOR can be used in a one-pot reaction as long as the reaction conditions follow the specifications for the FabRICATOR enzyme. It is important to have native reaction conditions for FabRICATOR to remain active.

 

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