Transglycosylation of IgG

TransGLYCIT is a platform technology that enables efficient and site-specific human IgG glycan remodeling or conjugation. With TransGLYCIT, antibodies with defined and homogenous glycoforms or site-specific azide-activation are generated using fast and robust enzymatic workflows.

The TransGLYCIT Remodeling formats generate a homogenous pool of antibodies carrying the G0, G1, G2 or G2S2 glycan profile with or without the core fucose, and the TransGLYCIT Azide Activation format generates azide-activated antibodies prepared for conjugation with any label of choice using click-chemistry (DOL = 4).
 

• Efficient and site-specific human IgG Fc N-glycan remodeling or conjugation
• Prepares human IgG with homogenous glycoprofiles, or azide activates human IgG with a reliable degree of labeling (DOL) of 4
• A rapid workflow – transglycosylation is completed within a few hours

Glycan remodeling of human IgG with the G0, G1, G2 or G2S2 glycoform.

TransGLYCIT Remodeling is a platform technology for efficient transglycosylation of native human IgG in a few hours. The robust workflow transglycosylates the antibody Fc N-glycans using enzymatic remodeling, resulting in a homogenous pool of antibodies carrying a defined glycoform. The technology is available in kits to generate antibodies carrying the G0, G1, G2 or G2S2 glycan profiles.

TransGLYCIT Remodeling contains sufficient material to transglycosylate 1 mg human IgG. The glycan remodeled sample will have the same degree of fucosylation as the original molecule. If you wish to remove core fucosylation, we recommend using TransGLYCIT Remodeling Afucosylated.
 

• Fast and efficient generation of human IgG with homogeneous glycoforms
• Facilitates functional analysis of specific glycan structures
• Reliable glycan remodeling achieved within a few hours

Unit Definition

TransGLYCIT transglycosylates ≥ 95% of 1 mg human IgG1 as measured by LC-MS.

Content and Storage

TransGLYCIT Remodeling contains:

• GlycINATOR Immobilized
• TransINATOR enzyme
• Oxazoline glycoform G0, G1, G2 or G2S2
• CaptureSelect™* spin column for purification

TransGLYCIT Remodeling is shipped cold, and the content should be stored at different temperatures upon arrival.
 
Do not freeze the GlycINATOR Immobilized or the CaptureSelect™ columns!

Glycan remodeling of human IgG with the G0, G1, G2 or G2S2 glycoform and core afucosylation.

TransGLYCIT Remodeling Afucosylated is a platform technology for efficient transglycosylation of native human IgG in a few hours. The robust workflow transglycosylates the antibody Fc N-glycans using enzymatic remodeling, resulting in a homogenous pool of antibodies carrying a defined glycoform (G0, G1, G2 or G2S2) with core afucosylation.

TransGLYCIT Remodeling Afucosylated is provided in kits containing sufficient material to transglycosylate 1 mg human IgG.
 

• Fast and efficient generation of human IgG with fully afucosylated homogeneous glycoforms
• Complete removal of core fucose to investigate its impact in functional assays
• Reliable glycan remodeling achieved within a few hours

Unit Definition

TransGLYCIT transglycosylates ≥ 95% of 1 mg human IgG1 as measured by LC-MS.

Content and Storage

TransGLYCIT Remodeling Afucosylated contains:

• GlycINATOR Immobilized/FucosEXO 16 Immobilized
• TransINATOR enzyme
• Oxazoline glycoform G0, G1, G2 or G2S2
• CaptureSelect™* spin column for purification

TransGLYCIT Remodeling Afucosylated is shipped cold, and the content should be stored at different temperatures upon arrival.
 
Do not freeze the GlycINATOR Immobilized/FucosEXO 16 Immobilized or the CaptureSelect™ columns!

Azide activation of human IgG1, IgG2 or IgG4.

TransGLYCIT Azide Activation enables efficient and site-specific conjugation at the Fc N-linked glycosylation sites of human IgG. Upon azide activation using TransGLYCIT Azide Activation, the antibody can be labeled with any alkyne-reactive click reagent of choice to a degree of labeling (DOL) of four labels per antibody (DOL = 4).

TransGLYCIT Azide Activation is available in kits containing sufficient material to azide activate 100 µg human IgG.

• Facilitates site-specific custom conjugation of human IgG with a consistent degree of labeling (DOL) of 4
• Higher DOL increases sensitivity for improved detection of low-level targets
• Fc-specific labeling preserves antigen binding-capacity
• Fast workflow for generation of azide-activated human IgG within a few hours

Unit Definition

TransGLYCIT Azide Activation transglycosylates ≥ 95% of 100 µg human IgG1 as measured by LC-MS.

Content and Storage

TransGLYCIT Azide Activation contains:

• GlycINATOR Immobilized
• TransINATOR enzyme
• Oxazoline glycoform Azide
• CaptureSelect™* spin column for purification

TransGLYCIT Azide Activation is shipped cold, and the content should be stored at different temperatures upon arrival.
 
Do not freeze the GlycINATOR Immobilized or the CaptureSelect™ columns!

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