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Specific Enrichment of O-glycosylated Proteins and Peptides

SmartEnzymes Book a Meeting Resources     Specific Enrichment of O-glycosylated Proteins and Peptides O-linked glycans are involved in several biological processes, including inhibiting infections caused by pathogens, inflammatory responses,…

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Generating Fab and Fc Antibody Fragments

GingisKHAN® Fab kit Site links All Applications All SmartEnzymes Book a Meeting Resources Intact Fab and Fc Antibody Fragments using FabALACTICA or GingisKHAN For applications involving intact Fab or Fc…

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VP Sales & Marketing

reporting and financial/budgeting process. Autonomous, initiative-taking, and results-oriented personality. Excellent verbal, written, and presentation communications skills. Education Ph.D. or M.Sc. in Biotechnology, Biomedicine, or other areas of the Life Sciences….

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FragIT kit

high specific affinity for the Fc. Both protein A and MabSelect Sure (GE) may have affinity for the Fab which may result in a lower recovery of the generated F(ab’)2…

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FabRICATOR MagIC Application

using FabRICATOR MagIC on a KingFisher instrument. Download BindIt protocol for KingFisher Duo Prime Download BindIt protocol for KingFisher Flex Download instructions for KingFisher Duo Prime & Flex workflows Figure…

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Fluorescent imaging

a crucial step in order to obtain high-quality imaging results. GlyCLICK delivers site-specific and quantitative labeling for conjugates with preserved antigen binding capacity for reproducible results in sensitive imaging applications….

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deGlycIT

B I did not get complete deglycosylation using deGlycIT, how can I improve digestion? First you could increase incubation time and increase temperature to 37°C. One explanation for incomplete deglycosylation…

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Immobilized GlycINATOR

on IgG with N-linked glycans in the Fab region? Yes, the GlycINATOR enzyme binds specifically to the Fc region and therefore will not deglycosylate N-linked glycans in the Fab region….

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GlyCLICK Characteristics

that utilizes glycan remodeling using the Fc-specific GlycINATOR®enzyme and combines it with click-chemistry for a stable and precise glycan-mediated antibody conjugation. The GlycINATOR enzyme digests all IgG glycoforms, including high-mannose,…

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Generation of Antibody Fragments

Reduction of non-specific binding from Fc Increased sensitivity in antigen detection by reduced steric hindrance from large epitopes Improved penetration into tissues for improved staining in immunohistochemistry (IHC) and imaging…

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