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Enzymes for O-glycans
OpeRATOR is an O-glycan-specific protease that digests mucin-type O-glycosylated proteins N-terminally of Ser and Thr glycosylation sites. Key Characteristics Digests native mucin-type O-glycosylated proteins 2h to overnight (16-18h) reaction Desialylation…
/products/enzymes-for-o-glycans/GlyCLICK Video
GlyCLICK® https://youtu.be/qFgLyfvhJ0Q GlyCLICK is a site-specific conjugation technology for IgG using enzymatic remodeling of the Fc-glycans and click-chemistry. It facilitates site-specific labeling of antibodies in a range of applications,…
/support/resources/video-glyclick/FabRICATOR MagIC
two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of…
/products/igg-proteases/fabricator/fabricator-magic/FabRICATOR
B What is the FabRICATOR MagIC? The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme….
/products/igg-proteases/fabricator/Place an Order – Genovis Inc.
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/support/place-an-order/genovis-inc/Unmasking charge variant of glycoproteins
icIEF Biosimilar Platform Method, https://www.proteinsimple.com/technical_library.html) Ampholyte solution: 1% Servalyts (0.5% 2-9, 0.5% 4-7) containing 6.4 M urea, 0.1% poloxamer 188, 10 mM IDA and 10 mM Arginine. pI markers: 4.05…
/applications/unmasking-charge-variant-of-glycoproteins/Place an Order – Genovis AB
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/support/place-an-order/genovis-ab/Download GlyCLICK Poster 2 Form Landing page
a stable and homogenous antibody conjugate. * SiteClick™ is provided under an intellectual property license from Life Technologies Corporation. The trademark SiteClick™ is the property of Life Technologies Corporation. Available…
/download-glyclick-poster-2-form/Antibody Fc-glycan Analysis
down workflow for mAb analysis using LC/MS A popular method for analyzing Fc glycosylation is hydrophilic interaction liquid chromatography high-performance liquid chromatography (HILIC-HPLC) on released N-glycans labelled with 2-aminobenzamide (2-AB;…
/applications/antibody-fc-glycan-analysis/Generating F(ab´)2 and Fc/2 Antibody Fragments
homogenous fragments with preserved immunoreactivity (Fig. 1). In addition, there is no risk for further degradation or over-digestion, a challenge otherwise commonly associated with other proteolytic enzymes like pepsin, papain…
/applications/generating-fab2-and-fc-2-antibody-fragments/