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Enzymes for O-glycans

OpeRATOR is an O-glycan-specific protease that digests mucin-type O-glycosylated proteins N-terminally of Ser and Thr glycosylation sites. Key Characteristics Digests native mucin-type O-glycosylated proteins 2h to overnight (16-18h) reaction Desialylation…

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GlyCLICK Video

GlyCLICK® https://youtu.be/qFgLyfvhJ0Q   GlyCLICK is a site-specific conjugation technology for IgG using enzymatic remodeling of the Fc-glycans and click-chemistry. It facilitates site-specific labeling of antibodies in a range of applications,…

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FabRICATOR MagIC

two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of…

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FabRICATOR

B What is the FabRICATOR MagIC? The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme….

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Place an Order – Genovis Inc.

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Unmasking charge variant of glycoproteins

icIEF Biosimilar Platform Method, https://www.proteinsimple.com/technical_library.html) Ampholyte solution: 1% Servalyts (0.5% 2-9, 0.5% 4-7) containing 6.4 M urea, 0.1% poloxamer 188, 10 mM IDA and 10 mM Arginine. pI markers: 4.05…

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Place an Order – Genovis AB

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Download GlyCLICK Poster 2 Form Landing page

a stable and homogenous antibody conjugate. * SiteClick™ is provided under an intellectual property license from Life Technologies Corporation. The trademark SiteClick™ is the property of Life Technologies Corporation. Available…

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Antibody Fc-glycan Analysis

down workflow for mAb analysis using LC/MS A popular method for analyzing Fc glycosylation is hydrophilic interaction liquid chromatography high-performance liquid chromatography (HILIC-HPLC) on released N-glycans labelled with 2-aminobenzamide (2-AB;…

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Generating F(ab´)2 and Fc/2 Antibody Fragments

homogenous fragments with preserved immunoreactivity (Fig. 1). In addition, there is no risk for further degradation or over-digestion, a challenge otherwise commonly associated with other proteolytic enzymes like pepsin, papain…

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