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Terms and Conditions

to manufacture, deliver or otherwise perform hereunder due to any cause beyond Seller’s reasonable control, including, without limitation, unsuccessful reactions, act of Buyer, embargo or other governmental act, regulation or…

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Removal of N-glycans from Glycoproteins

of N-glycans, leaving only the O-glycoforms.     Rapid N-glycan Removal using Immobilized PNGase F Denaturing To demonstrate the deglycosylation ability of Immobilized PNGase F on various substrates, a selection of glycoproteins…

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Resources

Biopharmaceuticals PEGS, 2019 GlycINATOR Therapeutic Antibodies Trimmed with GlycINATOR® Loses Antibody-Dependent Cell-mediated Cytotoxicity Antibody Industrial Symposium, 2018 GingisREX (RgpB) RgpB – an Arginine Specific Protease with Applications in Proteomics Analytical…

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Automated Antibody Subunit Analysis

attributes (CQAs) such as glycosylation, oxidation, and C-terminal lysine clipping. Such detailed knowledge of post-translational modifications (PTM) is required for the development and manufacturing of biopharmaceuticals. FabRICATOR-HPLC provides rapid on-column…

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Efficient Hydrolysis of Galactose

Galactosidase Activity on β1-3 Galactose from Etanercept GalactEXO also display enzymatic activity on β1-3 linked galactose present on O-glycosylated biopharmaceuticals such as etanercept. Etanercept was incubated with GalactEXO or galactosidases…

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Middle-level LC-MS Analysis of Mutated Antibodies

such antibodies requires specific enzymes to characterize properties such as monovalent binding, disulfide scrambling and paired Fc glycosylation. Traditional protocols include enzymatic digestion using papain and Lys-C, which require extensive…

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GlyCLICK Characteristics

    Site-specific Reliable performance Preserved affinity Versatile technology GlyCLICK Characteristics GlyCLICK is a site-specific conjugation technology for IgG using enzymatic remodeling of the Fc-glycan sites and click-chemistry. The key…

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GalactEXO

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.

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FabRICATOR-HPLC

B What is the FabRICATOR-HPLC? FabRICATOR-HPLC is an HPLC column with the FabRICATOR (IdeS) protease immobilized on a HPLC compatible matrix for automated antibody subunit generation….

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Immobilized PNGase F

B Which buffers can be used with the Immobilized PNGaseF column? The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral…

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