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Terms and Conditions

to manufacture, deliver or otherwise perform hereunder due to any cause beyond Seller’s reasonable control, including, without limitation, unsuccessful reactions, act of Buyer, embargo or other governmental act, regulation or…

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Intact and N-glycan Analysis of Glycoproteins

  Intact and N-glycan Analysis of Glycoproteins Page Specific Links Immobilized PNGase F and PNGase F Released Glycan Analysis Hydrolysis of N-glycans under Native Conditions Denaturing Conditions Speed up Deglycosylation PNGase F Activity…

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Automated Antibody Subunit Analysis

attributes (CQAs) such as glycosylation, oxidation, and C-terminal lysine clipping. Such detailed knowledge of post-translational modifications (PTM) is required for the development and manufacturing of biopharmaceuticals. FabRICATOR-HPLC provides rapid on-column…

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Efficient Hydrolysis of Galactose

Galactosidase Activity on β1-3 Galactose from Etanercept GalactEXO also display enzymatic activity on β1-3 linked galactose present on O-glycosylated biopharmaceuticals such as etanercept. Etanercept was incubated with GalactEXO or galactosidases…

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Resources

of Monoclonal Antibody Subunits Analytical Technologies Europe (CASSS), 2019 GlycINATOR Therapeutic Antibodies Trimmed with GlycINATOR® Loses Antibody-Dependent Cell-mediated Cytotoxicity Antibody Industrial Symposium, 2018 Immobilized SialEXO Complete and Rapid Desialylation of…

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PNGase F

B Which buffers can be used with the Immobilized PNGaseF column? The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral…

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Middle-level LC-MS Analysis of Mutated Antibodies

such antibodies requires specific enzymes to characterize properties such as monovalent binding, disulfide scrambling and paired Fc glycosylation. Traditional protocols include enzymatic digestion using papain and Lys-C, which require extensive…

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GlyCLICK Characteristics

    Site-specific Reliable performance Preserved affinity Versatile technology GlyCLICK Characteristics GlyCLICK is a site-specific conjugation technology for IgG using enzymatic remodeling of the Fc-glycan sites and click-chemistry. The key…

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GalactEXO

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.

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FabRICATOR-HPLC

B How many samples can I run through a column? The FabRICATOR-HPLC can run >400 samples of 10 ug of IgG….

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