SialEXO

SialEXO®

SialEXO is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for desialylation of up to 2 mg glycoprotein.

It is a sialidase mix for complete removal of sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins or released glycans.

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Detailed Information

SialEXO is a mixture of two sialidases for efficient desialylation of O- and N-glycosylated proteins. The sialidases in the mixture have unique activities and work simultaneously. One enzyme has a broad activity for α2-3, α2-6 and α2-8 linked sialic acids and the other one quickly hydrolyzes sialic acids with α2-3 linkage. Removes all sialic acids on native proteins within 2 h.

Hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 6.5 to 9.

The enzymes are derived from Akkermansia muciniphila and expressed in E. coli. Both enzymes contain a His-tag and the molecular weight of each enzyme is 43 kDa and 66 kDa, respectively.

Unit Definition

One unit of SialEXO hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

Supplied lyophilized in TBS  pH 7.6, with no preservatives added.
Shipped cold and should be stored at –20 °C upon arrival.
After reconstitution SialEXO is stable for at least 1 month at +4-8 °C.

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Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries regarding price for bulk quantities or licensing opportunities.

Documents

Instructions

Product Specifications

Safety Data Sheet

Certificate of Analysis

FAQ and Support

FAQ

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Yes, both enzymes are active under neutral conditions, preferably use 20 mM Tris pH 7.
Yes, SialEXO contains a 6x His-tag.

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Legal and Disclaimers

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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