SialEXO 23

SialEXO® 23

SialEXO 23 is supplied as a lyophilized powder with no preservatives added, and is available in 500 unit vials for desialylation of up to 0.5 mg glycoprotein.

SialEXO 23 is an α2-3 specific sialidase.

Detailed Information

In contrast to SialEXO which is a sialidase mix, SialEXO 23 is an α2-3 specific sialidase, allowing targeted analysis of α2-3 linked sialic acids only. Efficient α2-3 desialylation of O- and N-glycosylated proteins can be achieved within 1 h.

SialEXO 23 hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 7.0 to 9.0.

The enzyme is derived from Akkermansia muciniphila and expressed in E. coli. The enzyme has a His-tag and the molecular weight is 66 kDa.

Unit Definition

One unit of SialEXO 23 hydrolyzes α2-3 linked sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 7.5 at 37 °C for 1 h.

Content and Storage

SialEXO 23 is supplied lyophilized in TBS pH 7.6, with no preservatives added.
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
After reconstitution SialEXO 23 is stable for at least 1 month at +4-8 °C.

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Quotations

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Documents

Instructions

Product Specifications

Safety Data Sheet

Certificate of Analysis

FAQ and Support

FAQ

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Yes, both enzymes are active under neutral conditions, preferably use 20 mM Tris pH 7.
Yes, SialEXO contains a 6x His-tag.

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Legal and Disclaimers

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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