IgGZERO LE (Low Endotoxin) enzyme is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for deglycosylation of up to 2 mg IgG. This product formulation has low levels of endotoxins per vial (< 0.2 EU).

IgGZERO (EndoS) is an IgG-specific endoglycosidase acting on complex type N-glycans at the Fc-glycosylation site of IgG. The enzyme acts on the chitobiose core and leaves the core GlcNAc intact.

Detailed Information 

IgGZERO LE hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc intact on the Fc. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 110 kDa. 

IgGZERO is specific for N-glycans attached on the Fc-domain of IgGs, and hydrolyzes Fc-glycans of all human IgG subclasses and IgG from many other species, including mouse, rat, monkey, sheep, goat, cow, and horse. The reaction with IgGZERO is mild and runs fast under physiological conditions, with no need for co-factors or detergents.

The IgGZERO LE enzyme has limited activity on high-mannose and hybrid-type glycans. If you are interested in endoglycosidases that hydrolyze all IgG glycoforms, we recommend the GlycINATOR product family.

Storage and Stability

The IgGZERO LE enzyme is supplied lyophilized and reconstituted by addition of water. After reconstitution, IgGZERO LE is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.    

Unit Definition

One unit deglycosylates ≥ 95% of 1μg human IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.


How to Order

For details on ordering please visit How to order. Click add to cart to buy online.


Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.



Product Specifications


Certificate of Analysis

FAQ and Support

Popular FAQ


Find all FAQ here

Legal and Disclaimers

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.