product-logo-gingiskhanfabkit-center

GingisKHAN® Fab kit

GingisKHAN Fab kit is used for the preparation and purification of Fab fragments from human IgG1. The kit consists of lyophilized GingisKHAN enzyme and Reducing Agent for antibody digestion, and CH1-specific affinity spin columns.  

 

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity.

product-box-gingiskhan

Detailed Information

GingisKHAN Fab kit is used for rapid and easy preparation and purification of Fab fragments from human IgG1 antibodies. The GingisKHAN enzyme digests human IgG1 at a single site in the upper hinge (…KSCDK / THTCPPCP…), yielding intact Fab and Fc fragments. A second cleavage site on the Fc may appear if the N-glycans are removed. Mild reducing conditions (2 mM cysteine) is needed for enzymatic activity. Optimal activity is obtained at 37°C and pH 8.

The GingisKHAN Fab kit contains one vial of lyophilized GingisKHAN enzyme with 2000 units for digestion of up to 2 mg human IgG1, 5 vials of lyophilized GingisKHAN Reducing Agent and 4 microspin columns of CaptureSelect™* CH1 resin for affinity purification of the Fab fragment. Each microspin column includes sufficient material to purify up to 0.5 mg IgG.

The CaptureSelect™ IgG-CH1 affinity matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of Fab fragments independent of the light-chain isotype. Due to its unique selectivity for the CH1 domain, no co-purification of free light-chain contaminants will occur.

Stability and Storage

GingisKHAN Fab kit is shipped cold.

The GingisKHAN 2000 units and GingisKHAN Reducing Agent should be stored at -20°C upon arrival. The CaptureSelect™ IgG-CH1 columns should be stored in +4-8°C.

After reconstitution of the GingisKHAN enzyme, it is stable for 2 months at +4-8°C. Reconstituted GingisKHAN Reducing Agent should be used the same day as prepared, it should not be stored.

Unit Definition

One unit of GingisKHAN enzyme digests ≥ 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1h.

 

*Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

How to Order

For details on ordering please visit How to order. Click add to cart to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

Instructions

Product Specification

FAQ and Support

FAQ

There are not yet any FAQ's for this product. If you have a technical question, please submit a support case. 

Find all FAQ here

Patent and Disclaimer

GingisKHAN®

The trademark GingisKHAN® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic diagnostic use.

©2018 Genovis AB.