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GingisKHAN® Enzyme

GingisKHAN Enzyme is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for digestion of up to 2 mg human IgG1. It is provided together with 5 vials of lyophilized GingisKHAN Reducing Agent. 

 

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity.

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Detailed Information

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. GingisKHAN is purified from Porphyromonas gingivalis.

GingisKHAN is shipped together with 5 vials of lyophilized GingisKHAN Reducing Agent for convenient and reliable performance.

Storage and Stability

GingisKHAN is supplied as lyophilized powder. It is supplied with 5 vials of lyophilized GingisKHAN Reducing Agent (10x) containing 20 mM cysteine upon reconstitution.

GingisKHAN and GingisKHAN Reducing Agent are shipped cold and should be stored at -20°C upon arrival.

After reconstitution, the GingisKHAN enzyme is stable for at least 1 month +4-8°C. Reconstituted GingisKHAN Reducing Agent cannot be stored - use it freshly prepared.

Unit Definition

One unit cleaves ≥ 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1 hour.

How to Order

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Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

Instructions

Safety Data Sheet

FAQ and Support

FAQ

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Patent and Disclaimer


GingisKHAN®

The trademark GingisKHAN® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic diagnostic use.

©2018 Genovis AB.