GingisKHAN

GingisKHAN® Enzyme

GingisKHAN Enzyme is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for digestion of up to 2 mg human IgG1. It is provided together with 5 vials of lyophilized GingisKHAN Reducing Agent. 

 

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge. The enzyme requires only mild reduced condition for activity.

Detailed Information

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge (KSCDK / THTCPPC), generating intact Fab and Fc fragments. A second digestion site on the Fc may appear if the N-glycans are removed. GingisKHAN requires mild reducing conditions to be active, and optimal activity is obtained at 37°C and pH 8. GingisKHAN is purified from Porphyromonas gingivalis.

GingisKHAN is shipped together with 5 vials of lyophilized GingisKHAN Reducing Agent for convenient and reliable performance.

Storage and Stability

GingisKHAN is supplied as lyophilized powder. It is supplied with 5 vials of lyophilized GingisKHAN Reducing Agent (10x) containing 20 mM cysteine upon reconstitution.

GingisKHAN and GingisKHAN Reducing Agent are shipped cold and should be stored at -20°C upon arrival.

After reconstitution, the GingisKHAN enzyme is stable for at least 1 month +4-8°C. Reconstituted GingisKHAN Reducing Agent cannot be stored - use it freshly prepared.

Unit Definition

One unit cleaves ≥ 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1 hour.

How to Order

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Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

Instructions

Safety Data Sheet

Certificate of Analysis

FAQ and Support

Popular FAQ

Yes. GingisKHAN is provided lyophilized. After reconstitution, the enzyme can be used for up to 4 different antibodies. The digestion is performed in solution and you can prepare Fab fragments from 4 x 0.5 mg human IgG1. The purification step is done with Capture Select columns and 4 columns are provided in the kit.

Yes, GingisKHAN should be able to digest a Fc fusion protein if you have not fused the protein too close to the cleavage site and the Fc-part is from a human IgG1. GingisKHAN digests human IgG1 just above the hinge (…KSCDK / THTCPPCP…). If there are exposed Lysines in the fusion part, digestion might occur there as well.

No. GingisKHAN is a protease that digests at exposed Lysines with no secondary structure restrictions. At the recommended reaction conditions, GingisKHAN digestion above the hinge has only been confirmed on human IgG1 with the exposed lysine above the hinge, (…KSCDK / THTCPPCP…). No digestion has been confirmed on other subclasses of human IgG except for several digestion sites in human IgG3.

The enzyme is a cysteine protease that can be inhibited by alkylating agents such as iodoacetamide. There are also several other known inhibitors to this enzyme, like KYT-36.

Genovis provides a GingisKHAN Fab kit containing a specific Capture Select CH1 resin for further purification of the Fab. See this page for more information.

 

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