FabULOUS Fab kit

FabULOUS™ Fab kit

FabULOUS Fab kit contains one vial of FabULOUS enzyme with 2000 units for digestion of up to 2 mg mouse IgG, and 4 LC-Kappa (mur) microspin columns. Each column including sufficient material to purify up to 0.5 mg IgG.

FabULOUS (SpeB) is a unique recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region, producing a homogenous pool of Fab fragments. Together with the murine-specific affinity spin columns, the purification of prepared Fab fragments from mouse IgG is made possible.

Detailed Information

FabULOUS Fab kit is for rapid and easy preparation and purification of Fab fragments from mouse antibodies.

FabULOUS enzyme requires reduced condition for activity and the reaction is performed in the presence of 50mM Cysteine at neutral pH.

The CaptureSelect LC-Kappa (mur)* affinity matrix recognizes the LC-Kappa domain of murine IgG antibodies, which enables purification of Fab fragments.

Storage and Stability

FabULOUS Fab kit is shipped cold.

The FabULOUS enzyme should be stored at -20°C upon arrival.

CaptureSelect LC-Kappa (mur) resin* in MicroSpin columns should be stored at +4-8°C. The spin columns should not be frozen!

After reconstitution of the FabULOUS enzyme, it is stable for 1 months at +4-8°C.

Unit Definition

One unit digests ≥ 95% of 1µg human or mouse IgG when incubated in 10 mM Tris, 150 mM NaCl pH 7.4 at 37°C for 1 hour.


* Made with Thermo Scientific CaptureSelect resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Scientific and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

  • A1-PFK-020

    FabULOUS Fab kit mouse, 2mg mouse IgG

    A1-PFK-020870.00Add to cart

How to Order

For details on ordering please visit How to order. Click add to cart to buy online.


Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.



Product Specifications

Certificate of Analysis

FAQ and Support

Popular FAQ

Yes. There is the possibility to exchange the CaptureSelect LC-kappa (mur) columns to columns containing CaptureSelect LC-lambda (mouse). This resin is specific for mouse IgG. Please contact support@genovis.com if this is of interest.

No, FabULOUS Fab kit contains CaptureSelect LC-kappa (mur) spin columns with selective affinity for murine LC-kappa. If you need to generate pure Fab from human IgG1 you can use either FabALACTICA Fab Kit or GingisKHAN Fab Kit. If you have a different subclass of human IgG, please contact support@genovis.com for guidance.


Find all FAQ here