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FabULOUS Fab kit

FabULOUS Fab kit contains one vial of FabULOUS enzyme with 2000 units for digestion of up to 2 mg mouse IgG, and 4 LC-Kappa (mur) microspin columns. Each column including sufficient material to purify up to 0.5 mg IgG.

FabULOUS (SpeB) is a unique recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region, producing a homogenous pool of Fab fragments. Together with the murine-specific affinity spin columns, the purification of prepared Fab fragments from mouse IgG is made possible. 

Detailed Information

FabULOUS Fab kit is for rapid and easy preparation and purification of Fab fragments from mouse antibodies.

FabULOUS enzyme requires reduced condition for activity and the reaction is performed in the presence of 50mM Cysteine at neutral pH.

The CaptureSelect LC-Kappa (mur)* affinity matrix recognizes the LC-Kappa domain of murine IgG antibodies, which enables purification of Fab fragments.

Storage

FabULOUS Fab kit is shipped cold.

FabULOUS 2000u should be stored at -20°C upon arrival.

CaptureSelect LC-Kappa (mur) resin* in MicroSpin columns should be stored at +4-8°C. The spin columns should not be frozen!

After reconstitution of the FabULOUS enzyme, it is stable for 1 months at +4-8°C.

Unit definition

One unit digests ≥ 95% of 1µg human or mouse IgG when incubated in 10 mM Tris, 150 mM NaCl pH 7.4 at 37°C for 1 hour.

 

* Made with Thermo Scientific CaptureSelect resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Scientific and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

How to order

For details on ordering please visit How to order. Click add to cart to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Applications

Purified and homogenous Fab fragments can be used in many applications, including:

  • Study monovalent binding of Fab fragments to the antigen
  • Elimination of Fc-mediated effector functions
  • Reduced unspecific binding from Fc interactions 

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Figure 1. A) Intact monoclonal mouse IgG1 antibody. B) Analysis of fragments after FabULOUS Fab kit digestion. C) Eluted Fab fragments from the CaptureSelectTM LC-Kappa column. Digestion and purification were analyzed on RP-HPLC (Agilent 1290) using Waters Acquity UPLC® BEH300 C4.

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Figure 2. Non-reducing SDS-PAGE analysis of purified Fab fragments from monoclonal mIgG1 using FabULOUS Fab kit. Lane 1;intact mIgG1, Lane 2; digested mIgG1, Lane 3; flow through from CaptureSelect column, Lane 4; eluted Fab fragments, Lane 5; MW marker.

Documents

Instructions

Product specifications

FAQ and Support

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Patent and Disclaimer


FabULOUS®

The trademark FabULOUS® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic diagnostic use.