FabRICATOR References
- Drug-to-Antibody Ratio Estimation via Proteoform Peak Integration in the Analysis of Antibody–Oligonucleotide Conjugates with Orbitrap Fourier Transform Mass Spectrometry
2021-09-14
Konstantin O. Nagornov, Natalia Gasilova, Anton N. Kozhinov, Pasi Virta, Patrik Holm, Laure Menin, Victor J. Nesatyy, and Yury O. Tsybin - Characterization of N-Terminal Glutamate Cyclization in Monoclonal Antibody and Bispecific Antibody Using Charge Heterogeneity Assays and Hydrophobic Interaction Chromatography
2021-09-10
Mingyan Cao, Niluka De Mel, Jihong Wang, Conner Parthemore, Yang Jiao, Weimin Chen, Shihua Lin, Dengfeng Liu, Greg Kilby, Xiaoyu Chen - Bispecific antibody characterization by a combination of intact and site-specific/chain-specific LC/MS techniques
2021-09-03
Bastiaan L.Duivelshof, Alain Beck, Davy Guillarme, Valentina D'Atri - Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α
2021-08-17
Etienne Laborie, Victor Le-Minh, Thanh Duc Mai, Mehdi Ammar, Myriam Taverna & Claire Smadja - Evaluation of strategies for overcoming trifluoroacetic acid ionization suppression resulted in single-column intact level, middle-up, and bottom-up reversed-phase LC-MS analyses of antibody biopharmaceuticals
2021-05-12
Maria A. Khalikova, Laimonas Skarbalius, Denis K. Naplekov, Siddharth Jadeja, František Švec, Juraj Lenčo - In-capillary (electrophoretic) digestion-reduction-separation: A smart tool for middle-up analysis of mAb
2021-05-03
Meriem Dadouch, Yoann Ladner, Claudia Bich, Jérôme Montels, Jacques Morel, Cherine Bechara, Catherine Perrin - Chromatographic and Adsorptive Behavior of a Bivalent Bispecific Antibody and Associated Fragments
2021-04-27
Lucas K. Kimerer, Ben Niu, Timothy M. Pabst, Weiguo Zhai, Alan K. Hunter, Giorgio Carta - Automated and Faster Affinity Capture Method for Biotransformation Assessment of Site-Specific Antibody Drug Conjugates
2021-03-22
Aarti Jashnani, Srikanth Kotapati, Madhura Deshpande, Sayumi Yamazoe, Pavel Strop, Arvind Rajpal and Gavin Dollinger - Application of middle-down approach in quantitation and catabolite identification of protein by LC–high-resolution mass spectrometry
2021-03-15
Lijuan Kang, Shengsheng Xu, Yongle Pang, Tom Kirchner, Yue-mei Zhang, Wilson Edwards, Raul Camacho, Lisa Norquay, Naidong Weng & Wenying Jian - Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry
2021-02-24
Honorine Lardeux, Bastiaan L.Duivelshof, OlivierColas, AlainBeck, David V.McCalley, DavyGuillarme, ValentinaD’Atri - Electron Transfer Dissociation Parameter Optimization Using Design of Experiments Increases Sequence Coverage of Monoclonal Antibodies
2021-02-17
Milos Cejkov, Tyler Greer, Reid O’Brien Johnson, Xiaojing Zheng and Ning Li - Microdroplet Ultrafast Reactions Speed Antibody Characterization
2021-02-05
Pengyi Zhao, Harsha P. Gunawardena, Xiaoqin Zhong, Richard N. Zare and Hao Chen - C-terminal lysine clipping of IgG1: impact on binding to human FcγRIIIa and neonatal Fc receptors
2021-01-18
Valegh Faid, Yann Leblanc, Marie Berger, Alexander Seifert, Nicolas Bihoreau, Guillaume Chevreux - Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond network
2020-11-17
Elke Prade, Anne Zeck, Fabian Stiefel, Andreas Unsoeld, David Mentrup, Erik Arango-Gutierrez, Ingo H. Gorr - Integrating Intact Mass Analysis and Middle-Down Mass Spectrometry Approaches to Effectively Characterize Trastuzumab and Adalimumab Structural Heterogeneity
2020-10-29
Wenwen Zhu, Menglin Li, Jinlan Zhang - Use of Ultra-short Columns for Therapeutic Protein Separations, Part 2: Designing the Optimal Column Dimension for Reversed- Phase Liquid Chromatography
2020-10-07
Szabolcs Fekete, Amarande Murisier, Jennifer M. Nguyen, Michael J. Bolton, Jonathan Belanger, Alain Beck, Jean-Luc Veuthey, Kevin Wyndham, Matthew A. Lauber, and Davy Guillarme - Selectivity over coverage in de novo sequencing of IgGs
2020-10-06
Maurits A. den Boer, Jean-Francois Greisch, Sem Tamara, Albert Bondt, Albert J. R. Heck - Towards automation of Collision Induced Unfolding experiments through online Size Exclusion Chromatography coupled to native Mass Spectrometry
2020-09-04
Evolène Deslignière, Anthony Ehkirch, Thomas Botzanowski, Alain Beck, Oscar Hernandez-Alba, and Sarah Cianférani - Improved N- and C-terminal sequencing of proteins by combining positive and negative ion MALDI in-source decay mass spectrometry
2020-08-17
Simone Nicolardi, David Peter Alexander Kilgour, Yuri E.M. van der Burgt, Manfred Wuhrer - IgG-cleaving endopeptidase enables in vivo gene therapy in the presence of anti-AAV neutralizing antibodies
2020-06-01
Christian Leborgne, Elena Barbon, Jeffrey M. Alexander, Hayley Hanby, Sandrine Delignat, Daniel M. Cohen, Fanny Collaud, Saghana Muraleetharan, Dan Lupo, Joseph Silverberg, Karen Huang, Laetitia van Wittengerghe, Béatrice Marolleau, Adeline Miranda, Anna Fabiano, Victoria Daventure, Heena Beck, Xavier M. Anguela, Giuseppe Ronzitti, Sean M. Armour, Sebastien Lacroix-Desmazes & Federico Mingozzi - Middle Level IM–MS and CIU Experiments for Improved Therapeutic Immunoglobulin Subclass Fingerprinting
2020-05-26
Thomas Botzanowski, Oscar Hernandez-Alba, Martine Malissard, Elsa Wagner-Rousset, Evolène Deslignière, Olivier Colas, Jean-François Haeuw, Alain Beck, and Sarah Cianférani - Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms
2020-05-21
Melanie Ramberger, Antonio Berretta, Jeanne M M Tan, Bo Sun, Sophia Michael, Tianrong Yeo, Jakob Theorell, Rachael Bashford-Rogers, Sofija Paneva, Victoria O’Dowd, Neesha Dedi, Sarfaraj Topia, Robert Griffin, Jorge Ramirez-Franco, Oussama El Far, Stéphanie Baulac, Maria I Leite, Arjune Sen, Alexander Jeans, David McMillan, Diane Marshall, Daniel Anthony, Daniel Lightwood, Patrick Waters, Sarosh R Irani - Generation and validation of structurally defined antibody–siRNA conjugates
2020-04-29
Alex R Nanna, Alexander V Kel’in, Christopher Theile, Justin M Pierson, Zhi Xiang Voo, Ashish Garg, Jayaprakash K Nair, Martin A Maier, Kevin Fitzgerald, Christoph Rader - Monitoring CD8a+ T Cell Responses to Radiotherapy and CTLA-4 Blockade Using [64Cu]NOTA-CD8a PET Imaging
2020-02-21
Lotte K. Kristensen, Camilla Christensen, Maria Z. Alfsen, Sigrid Cold, Carsten H. Nielsen & Andreas Kjaer - Combination of intact, middle-up and bottom-up levels to characterize 7 therapeutic monoclonal antibodies by capillary electrophoresis – Mass spectrometry
2020-01-23
Jérémie Giorgetti, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François, - Middle-level IM-MS and CIU experiments for improved therapeutic immunoglobulin isotype fingerprinting
2020-01-21
Thomas Botzanowski, Oscar Hernandez-Alba, Martine Malissard, Elsa Wagner-Rousset, Evolène Deslignière, Olivier Colas, Jean-François Haeuw, Alain Beck, Sarah Cianférani - Minimal B Cell Extrinsic IgG Glycan Modifications of Pro- and Anti-Inflammatory IgG Preparations in vivo
2020-01-09
Anja Schaffert, Maja Hanic´, Mislav Novokmet, Olga Zaytseva, Jasminka Krištic´, Anja Lux, Lars Nitschke, Matthias Peipp, Marija Pezer, René Hennig, Erdmann Rapp, Gordan Lauc, Falk Nimmerjahn - Implementation of a High-Resolution Liquid Chromatography–Mass Spectrometry Method in Quality Control Laboratories for Release and Stability Testing of a Commercial Antibody Product
2019-12-23
Izabela Sokolowska, Jingjie Mo,Fatie Rahimi Pirkolachahi, Carol McVean, Lars A. T. Meijer, Linda Switzar, Crina Balog, Michael J. Lewis, Ping Hu - Universal Affinity Capture Liquid Chromatography-Mass Spectrometry Assay for Evaluation of Biotransformation of Site-Specific Antibody Drug Conjugates in Preclinical Studies
2019-12-20
Srikanth Kotapati, David Passmore, Sayumi Yamazoe, Rajesh Kishore Kumar Sanku, Qiang Cong, Yam B. Poudel, Naidu S. Chowdari, Sanjeev Gangwar, Chetana Rao, Vangipuram S. Rangan, Pina M. Cardarelli, Shrikant Deshpande, Pavel Strop, Gavin Dollinger, and Arvind Rajpal - Analysis of cetuximab N-Glycosylation using multiple fractionation methods and capillary electrophoresis mass spectrometry
2019-12-07
Csaba Váradi, Craig Jakes, Jonathan Bones - Strong Cation Exchange Ultra-Performance Liquid Chromatography Coupled to UV and MS Detectors Allows Robust and Reproducible Characterization of mAb and ADC Charge Variants
2019-12-05
Michal Kliman, Arnie Aistars, Yutaka Matsuda, Brian A. Mendelsohn - Comparing different domains of analysis for the characterisation of N-Glycans on monoclonal antibodies
2019-11-29
Sara Carillo, Raquel Pérez-Robles, CraigJakes, Meire Ribeiro da Silva, Silvia Millán Martín, Amy Farrell, Natalia Navas, Jonathan Bones - TR1801-ADC: A highly potent cMet antibody-drug conjugate with high activity in patient-derived xenograft models of solid tumors
2019-11-14
Marco Gymnopoulos, Oscar Betancourt, Vincent Blot, Ryo Fujita, Diana Galvan, Vincent Lieuw, Sophie Nguyen, Jeanette Snedden, Christine Stewart, Jose Villicana, Jon Wojciak, Eley Wong, Raul Pardo, Neki Patel, Francois D’Hooge, Balakumar Vijayakrishnan, Conor Barry, John A. Hartley, Philip W. Howard, Roland Newman, Julia Coronella - Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh resolution MALDI FT-ICR mass spectrometry
2019-10-21
Christoph Gstöttner, Dietmar Reusch, Markus Haberger, Irina Dragan, Peter van Veelen, David P. A. Kilgour, Yury O. Tsybin, Yuri E.M. van der Burgt, Manfred Wuhrer & Simone Nicolardi - Unambiguous Sequence Characterization of a Monoclonal Antibody in a Single Analysis Using a Nonspecific Immobilized Enzyme Reactor
2019-10-04
Joshua Hinkle, Robert Anthony D'Ippolito, Maria C. Panepinto, Weihan Wang, Dina L. Bai, Jeffrey Shabanowitz, and Donald F. Hunt - Susceptibility of protein therapeutics to spontaneous chemical modifications by oxidation, cyclization, and elimination reactions
2019-10-01
Luigi Grassi, Chiara Cabrele - A generic method for intact and subunit level characterization of mAb charge variants by native mass spectrometry
2019-09-23
Y. Leblanc, V. Faid, M.A. Lauber, Q. Wang, N. Bihoreau, G. Chevreux - Middle-Down Multi-Attribute Analysis of Antibody- Drug Conjugates with Electron Transfer Dissociation
2019-08-23
Bifan Chen, Ziqing Lin, Yanlong Zhu, Yutong Jin, Eli Larson, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne Pritts, and Ying Ge - In Vivo Surveillance and Elimination of Teratoma-forming Human Embryonic Stem Cells with Monoclonal Antibody 2448 targeting Annexin A2
2019-08-07
Heng Liang Tan, Bao Zhu Tan, Winfred Xi Tai Goh, Simeon Cua, Andre Choo - Forced Degradation Testing as Complementary Tool for Biosimilarity Assessment
2019-07-21
Yan Felix Karl Dyck, Daniel Rehm, Jan Felix Joseph, Karsten Winkler, Volker Sandig, Wolfgang Jabs, Maria Kristina Parr - Utility of High Resolution NMR Methods to Probe the Impact of Chemical Modifications on Higher Order Structure of Monoclonal Antibodies in Relation to Antigen Binding
2019-07-01
Subhabrata Majumder, Andrew Saati, Shibu Philip, Lucy L. Liu, Elaine Stephens, Jason C. Rouse, Arun Alphonse Ignatius - Using bispecific antibodies in forced degradation studies to analyze the structure–function relationships of symmetrically and asymmetrically modified antibodies
2019-06-04
Adam R. Evans, Michael T. Capaldi, Geetha Goparaju, David Colter, Frank F. Shi, Sarah Aubert, Lian-Chao Li, Jingjie Mo, Michael J. Lewis, Ping Hu, Pedro Alfonso & Promod Mehndiratta - Confident monoclonal antibody sequence verification by complementary LC-MS techniques
2019-05-23
Amy Farrell, Sara Carillo, Jonathan Bones, Kai Scheffler, Ken Cook - Two sequential layers of antibody‐mediated control of Legionella pneumophila infection
2019-05-10
Stefan S. Weber, Diana Stoycheva, Falk Nimmerjahn, Annette Oxenius - Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 2: evaluation of recent stationary phases
2019-05-09
Amarande Murisiera, Evelin Farsang, Krisztián Horváthb, Matthew Lauberc, Alain Beck, Davy Guillarmea, Szabolcs Feketea - Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
2019-04-24
GE Bergdahl, M Hedström, B Mattiasson - Chromatographic behavior of bivalent bispecific antibodies on cation exchange columns. II. Biomolecular perspectives
2019-04-09
Lucas K. Kimerer, Timothy M. Pabst, Alan K. Hunter, Giorgio Carta - Computer-aided gradient optimization of hydrophilic interaction liquid chromatographic separations of intact proteins and protein glycoforms
2019-04-03
Guusje van Schaick Bob W.J. Pirok Rob Haselberg Govert W. Somsen Andrea F.G. Gargano - Application of a label-free and domain-specific free thiol method in monoclonal antibody characterization
2019-03-25
Yi Pu, Yunqiu Chen, Tai Nguyen, Chong-Feng Xu, Li Zang, Zoran Sosic, Tyler Carlage - N-glycans of complex glycosylated biopharmaceuticals and their impact on protein clearance
2019-03-21
Fabian Higel, Theresa Sandl, Chi-Ya Kao, Nicole Pechinger, Fritz Sörgel, Wolfgang Friess, Florian Wolschina, Andreas Seidl - Longitudinal Analysis Reveals Early Development of Three MPER-Directed Neutralizing Antibody Lineages from an HIV-1-Infected Individual
2019-03-19
Shelly J. Krebs, Young D. Kwon, Chaim A. Schramm, ..., Merlin L. Robb, Peter D. Kwong, Nicole A. Doria-Rose - IgG Charge: Practical and Biological Implications
2019-03-14
Danlin Yang, Rachel Kroe-Barrett, Sanjaya Singh, Thomas Laue - MHC class II proteins mediate cross-species entry of bat influenza viruses
2019-02-20
Umut Karakus, Thiprampai Thamamongood, Kevin Ciminski, Wei Ran, Sira C. Günther, Marie O. Pohl, Davide Eletto, Csaba Jeney, Donata Hoffmann, Sven Reiche, Jan Schinköthe, Reiner Ulrich, Julius Wiener, Michael G. B. Hayes, Max W. Chang, Annika Hunziker, Emilio Yángüez, Teresa Aydillo, Florian Krammer, Josua Oderbolz, Matthias Meier, Annette Oxenius, Anne Halenius, Gert Zimmer, Christopher Benner, Benjamin G. Hale, Adolfo García-Sastre, Martin Beer, Martin Schwemmle & Silke Stertz - Persistent Antibody Clonotypes Dominate the Serum Response to Influenza over Multiple Years and Repeated Vaccinations
2019-02-19
Jiwon Lee, Philipp Paparoditis, Andrew P. Horton, Alexander Frühwirth, Jonathan R.McDaniel, Jiwon Jung, Daniel R. Boutz, Dania A. Hussein, Yuri Tanno, Leontios Pappas, Gregory C. Ippolito, Davide Corti, Antonio Lanzavecchia, GeorgeGeorgiou - Tuning selectivity in cation-exchange chromatography applied for monoclonal antibody separations, part 1: Alternative mobile phases and fine tuning of the separation
2019-02-19
Evelin Farsang, Amarande Murisier, Krisztián Horvátha, Alain Beck, Róbert Kormányd, Davy Guillarme, Szabolcs Fekete - Direct quantitation of therapeutic antibodies for pharmacokinetic studies using immuno-purification and intact mass analysis
2019-02-08
Lisa A Vasicek, Xin Zhu, Daniel S Spellman & Kevin P Bateman - Fc Sialylation Prolongs Serum Half-Life of Therapeutic Antibodies
2019-01-25
Mathilde Bas, Aurélie Terrier, Emilie Jacque, Aurélie Dehenne, Virginie Pochet-Béghin, Cécile Beghin, Anne-Sophie Dezetter, Gilles Dupont, Anaïs Engrand, Benjamin Beaufils, Philippe Mondon, Nathalie Fournier, Christophe de Romeuf, Sylvie Jorieux, Alexandre Fontayne, Lennart T. Mars and Céline Monnet - A generic workflow for the characterization of therapeutic monoclonal antibodies—application to daratumumab
2019-01-07
Bastiaan L. Duivelshof, Szabolcs Fekete, Davy Guillarme, Valentina D’Atri - Structural Analysis of Monoclonal Antibodies by Ultrahigh Resolution MALDI In-Source Decay FT-ICR Mass Spectrometry
2018-12-20
Yuri E.M. van der Burgt, David P. A. Kilgour, Yury O. Tsybin, Kristina Srzentic,́ Luca Fornelli, Alain Beck, Manfred Wuhrer, and Simone Nicolardi - A generic workflow for the characterization of therapeutic monoclonal antibodies—application to daratumumab
2018-12-19
Bastiaan L. Duivelshof & Szabolcs Fekete & Davy Guillarme & Valentina D’Atri - Orthogonal Middle-up Approaches for Characterization of the Glycan Heterogeneity of Etanercept by Hydrophilic Interaction Chromatography Coupled to High-Resolution Mass Spectrometry
2018-12-04
Valentina D’Atri, Lucie Novaḱova, Szabolcs Fekete, Dwight Stoll, Matthew Lauber, Alain Beck, and Davy Guillarme - Fast and Simple Qualitative/Semi‐Quantitative Analysis of Monoclonal Antibody Mixtures Using Liquid Chromatography–Electrospray Triple Time‐of‐Flight Mass Spectrometry
2018-10-15
Jin‐Ju Byeon Min‐Ho Park Seok‐Ho Shin Young G. Shin - Ultrasensitive Characterization of Charge Heterogeneity of Therapeutic Monoclonal Antibodies Using Strong Cation Exchange Chromatography Coupled to Native Mass Spectrometry
2018-10-03
Yuetian Yan, Anita Liu, Shunhai Wang, Thomas J. Daly, Ning Li - Comprehensive characterization of monoclonal antibody by Fourier transform ion cyclotron resonance mass spectrometry
2018-09-19
Yutong Jin, Ziqing Lin, Qingge Xu, Cexiong Fu, Zhaorui Zhang, Qunying Zhang, Wayne A. Pritts, Ying Ge - Chemical Structure and Composition of Major Glycans Covalently Linked to Therapeutic Monoclonal Antibodies by Middle-down NMR
2018-08-13
Jiangnan Peng, Sharadrao M Patil, David A. Keire, Kang Chen - Heterogeneity assessment of antibody-derived therapeutics at the intact and middle-up level by low-flow sheathless capillary electrophoresis-mass spectrometry
2018-08-12
Rob Haselberg, Thomas De Vijlder, Raimond Heukers, Martine J. Smit, Edwin P. Romijn, Govert W. Somsen, Elena Domínguez-Vega - Systematic analysis of monoclonal antibodies against Ebola virus GP defines features that contribute to protection
2018-08-09
Erica Ollmann Saphire, Sharon L. Schendel, Marnie L. Fusco, Karthik Gangavarapu, Bronwyn M. Gunn, Anna Z. Wec, Peter J. Halfmann, Jennifer M. Brannan, Andrew S. Herbert, Xiangguo Qiu, Kshitij Wagh, Shihua He, Elena E. Giorgi, James Theiler, Kathleen B. J. Pommert, Tyler B. Krause, Hannah L. Turner, Charles D. Murin, Jesper Pallesen, Edgar Davidson, Rafi Ahmed, M. Javad Aman, Alexander Bukreyev, Dennis R. Burton, James E. Crowe Jr., Carl W. Davis, George Georgiou, Florian Krammer, Christos A. Kyratsous, Jonathan R. Lai, Cory Nykiforuk, Michael H. Pauly, Pramila Rijal, Ayato Takada, Alain R. Townsend, Viktor Volchkov, Laura M. Walker, Cheng-I Wang, Larry Zeitlin, Benjamin J. Doranz, Andrew B. Ward, Bette Korber, Gary P. Kobinger, Kristian G. Andersen, Yoshihiro Kawaoka, Galit Alter, Kartik Chandran, John M. Dye - A Role for Fc Function in Therapeutic Monoclonal Antibody-Mediated Protection against Ebola Virus
2018-08-08
Bronwyn M. Gunn, Wen-Han Yu, Marcus M. Karim, Jennifer M. Brannan, Andrew S. Herbert, Anna Z. Wec, Peter J. Halfmann, Marnie L. Fusco, Sharon L. Schendel, Karthik Gangavarapu, Tyler Krause, Xiangguo Qiu, Shinhua He, Jishnu Das, Todd J. Suscovich, Jonathan Lai, Kartik Chandran, Larry Zeitlin, James E. Crowe, Jr., Douglas Lauffenburger, Yoshihiro Kawaoka, Gary P. Kobinger, Kristian G. Andersen, John M. Dye, Erica Ollmann Saphire, Galit Alter - Rapid charge variant analysis of monoclonal antibodies to support lead candidate biopharmaceutical development
2018-07-29
Anne Trappe, Florian Füssl, Sara Carillo, Izabela Zaborowska, Paula Meleady, Jonathan Bones - Characterization and analysis of scFv-IgG bispecific antibody size variants
2018-07-25
Mingyan Cao, Chunlei Wang, Wai Keen Chung, Dana Motabar, Jihong Wang, Elizabeth Christian, Shihua Lin, Alan Hunter, Xiangyang Wang & Dengfeng Liu - Accurate Sequence Analysis of a Monoclonal Antibody by Top- Down and Middle-Down Orbitrap Mass Spectrometry Applying Multiple Ion Activation Techniques
2018-06-12
Luca Fornelli, Kristina Srzentić, Romain Huguet, Christopher Mullen, Seema Sharma, Vlad Zabrouskov, Ryan T. Fellers, Kenneth R. Durbin, Philip D. Compton, Neil L. Kelleher - Monoclonal antibody sequence assessment using a hybrid quadrupole-Orbitrap mass spectrometer
2018-05-31
Amy Farrell, Sara Carillo, Kai Scheffler, Ken Cook, Jonathan Bones - Development of Comprehensive Online Two-Dimensional Liquid Chromatography/Mass Spectrometry Using Hydrophilic Interaction and Reversed-Phase Separations for Rapid and Deep Profiling of Therapeutic Antibodies
2018-04-04
Dwight R Stoll, David Christopher Harmes, Gregory O. Staples, Oscar G. Potter, Carston T. Dammann, Davy Guillarme, and Alain Beck - Characterization of recombinant monoclonal antibody variants detected by hydrophobic interaction chromatography and imaged capillary isoelectric focusing electrophoresis
2018-03-30
Cory King, Rekha Patel, Gomathinayagam Ponniah, Christine Nowak, Alyssa Neill, Zhenyu Gu, Hongcheng Liu - Hydrophilic Auristatin Glycoside Payload Enables Improved Antibody-Drug Conjugate Efficacy and Biocompatibility
2018-03-22
Tero Satomaa, Henna Pynnönen, Anja Vilkman, Titta Kotiranta, Virve Pitkänen, Annamari Heiskanen, Bram Herpers, Leo S. Price, Jari Helin and Juhani Saarinen - Targeting of embryonic annexin A2 expressed on ovarian and breast cancer by the novel monoclonal antibody 2448
2018-03-02
Simeon Cua, Heng Liang Tan, Wey Jia Fong, Angela Chin, Ally Lau, Vanessa Ding, Zhiwei Song, Yuansheng Yang and Andre Choo - A Polar Sulfamide Spacer Significantly Enhances the Manufacturability, Stability, and Therapeutic Index of Antibody–Drug Conjugates
2018-02-20
Jorge M. M. Verkade, Marloes A. Wijdeven, Remon van Geel, Brian M. G. Janssen, Sander S. van Berkel and Floris L. van Delft - Characterization of an antibody-drug conjugate by hydrophilic interaction chromatography coupled to mass spectrometry
2018-02-17
Valentina D’Atri, Szabolcs Fekete, Dwight Stoll, Matthew Lauber, Alain Beck, Davy Guillarme - Practical approaches for overcoming challenges in heightened characterization of antibody-drug conjugates with new methodologies and ultrahigh-resolution mass spectrometry
2018-02-02
Olga V. Friese, Jacquelynn N. Smith, Paul W. Brown & Jason C. Rouse - Parsimonious Charge Deconvolution for Native Mass Spectrometry
2018-01-29
Marshall Bern, Tomislav Caval, Yong J Kil, Wilfred Tang, Christopher Becker, Eric Carlson, Doron Kletter, K. Ilker Sen, Nicolas Galy, Dominique Hagemans, Vojtech Franc, and Albert J.R. Heck - IdeS-cleaved mAb subunit analysis with LC-HRAM-MS: a quick and accurate comparison of biosimilar and originator biotherapeutics
2018-01-01
Sara Carillo, Craig Jakes, Izabela Zaborowska, Jonathan Bones, National Institute for Bioprocessing Research and Training (NIBRT) - Subunits analysis approach for the determination of fucosylation levels in monoclonal antibodies using LC-HRAM-MS
2018-01-01
Craig Jakes, Sara Carillo, Izabela Zaborowska, and Jonathan Bones National Institute for Bioprocessing Research and Training (NIBRT) - Middle-up analysis of monoclonal antibodies after combined IgdE and IdeS hinge proteolysis: Investigation of free sulfhydryls
2017-11-21
Valegh Faid, Yann Leblanc, Nicolas Bihoreau, Guillaume Chevreux - Protein de novo sequencing by top-down and middle-down MS/MS: Limitations imposed by mass measurement accuracy and gaps in sequence coverage
2017-11-20
Lidong He, Chad R. Weisbrod, Alan G. Marshall - Massive immune response against IVIg interferes with response against other antigens in mice: A new mode of action?
2017-10-12
Laetitia Sordé, Sebastian Spindeldreher, Ed Palmer, Anette Karle - Identification and Quantification of Signal Peptide Variants in an IgG1 Monoclonal Antibody Produced in Mammalian Cell Lines
2017-08-19
Yunping Huang, Jinmei Fu, Richard Ludwig, Li Tao, Jacob Bongers, Li Ma, Ming Yao, Mingshe Zhu, Tapan Das, Reb Russell - Preclinical Characterization of CTL-1, A Biosimilar Anti-EGFR Monoclonal Antibody for Cetuximab
2017-08-10
Peisheng Hu, Ryan Gra, Long Zheng, Leslie A Khawli and Alan L Epstein - A Generic HPLC Method for Absolute Quantification of Oxidation in Monoclonal Antibodies and Fc-Fusion Proteins Using UV and MS Detection
2017-06-28
Christof Regl, Therese Wohlschlager, Johann Holzmann and Christian G. Huber - Current possibilities of liquid chromatography for the characterization of antibody-drug conjugates
2017-06-24
Balázs Bobály, Sandrine Fleury-Souverain, Alain Beck, Jean-Luc Veuthey, Davy Guillarme, Szabolcs Fekete - Discovery and Characterization of Histidine Oxidation Initiated Cross-links in an IgG1 Monoclonal Antibody
2017-06-21
Chong-Feng Xu, Yunqiu Chen, Linda Yi, Tim Brantley, Brad Stanley, Zoran Sosic, and Li Zang - Protocols for the analytical characterization of therapeutic monoclonal antibodies. II – Enzymatic and chemical sample preparation
2017-06-19
Balazs Bobaly, Valentina D’Atri, Alexandre Goyon, Olivier Colas, Alain Beck, Szabolcs Fekete, Davy Guillarme - Analysis of recombinant monoclonal antibodies in hydrophilic interaction chromatography: A generic method development approach
2017-06-11
Balázs Bobály, Valentina D’Atri, Alain Beck, Davy Guillarme, Szabolcs Fekete - Stable Isotope Quantitative N-Glycan Analysis by Liquid Separation Techniques and Mass Spectrometry
2017-05-14
Stefan Mittermayr, Simone Albrecht, Csaba Váradi, Silvia Millán-Martín, and Jonathan Bones - High resolution top-down experimental strategies on the Orbitrap platform
2017-03-31
Kai Scheffler, Rosa Viner, Eugen Damoc - Orthogonal liquid chromatography–mass spectrometry methods for the comprehensive characterization of therapeutic glycoproteins, from released glycans to intact protein level
2017-03-27
Eric Largy, Fabrice Cantais, Géry Van Vyncht, Alain Beck, Arnaud Delobel - Comparability study of Rituximab originator and follow-on biopharmaceutical
2017-03-19
Othman Montacir, Houda Montacir, Murat Eravci, Andreas Springer, Stephan Hinderlich, Amirhossein Saadati, Maria Kristina Parr - Analysis of Monoclonal Antibodies in Human Serum as a Model for Clinical Monoclonal Gammopathy by Use of 21 Tesla FT-ICR Top-Down and Middle-Down MS/MS
2017-02-28
Lidong HeLissa C. AndersonDavid R. BarnidgeDavid L. MurrayChristopher L. HendricksonAlan G. Marshall - Top-down and middle-down approach by fraction collection enrichment using off-line capillary electrophoresis – mass spectrometry coupling: Application to monoclonal antibody F c/2 charge variants
2017-02-27
Michael Biacchi, Nassur Said, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François, - Development of a fast workflow to screen the charge variants of therapeutic antibodies
2017-02-26
Elsa Wagner-Rousset, Szabolcs Fekete, Laura Morel-Chevillet, Olivier Colas, Nathalie Corva ̈ıa, Davy Guillarme, Alain Beck - Rapid assessment of oxidation via middle-down LCMS correlates with methionine side-chain solvent-accessible surface area for 121 clinical stage monoclonal antibodies
2017-02-14
Rong Yang, Tushar Jain, Heather Lynaugh, R. Paul Nobrega, Xiaojun Lu, Todd Boland, Irina Burnina, Tingwan Sun, Isabelle Caffry, Michael Brown, Xiaoyong Zhi, Asparouh Lilov & Yingda Xu - Subunit mass analysis for monitoring antibody oxidation
2017-01-20
Izabela Sokolowska, Jingjie Mo, Jia Dong, Michael J. Lewis, Ping Hu - Highly sensitive glycosylamine labelling of O-glycans using non-reductive β-elimination
2017-01-14
Kenichiro Furuki, Toshimasa Toyo’oka & Kazutoshi Ban - Guiding bispecific monovalent antibody formation through proteolysis of IgG1 single-chain
2017-01-05
Nazzareno Dimasi, Ryan Fleming, Kris F. Sachsenmeier, Binyam Bezabeh, Carl Hay, Jincheng Wu, Erin Sult, Saravanan Rajan, Li Zhuang, Peter Cariuk, Andrew Buchanan, Michael A. Bowen, Herren Wu & Changshou Gao - Anti-Hinge Antibodies Recognize IgG Subclass- and Protease-Restricted Neoepitopes
2017-01-01
Willem J. J. Falkenburg, Dirkjan van Schaardenburg, Pleuni Ooijevaar-de Heer, Michel W. P. Tsang-A-Sjoe,Irene E. M. Bultink, Alexandre E. Voskuyl, Arthur E. H. Bentlage, Gestur Vidarsson, Gertjan Wolbink, and Theo Rispens - Characterization of charge variants of a monoclonal antibody using weak anion exchange chromatography at subunit levels
2016-12-23
G. Ponniah, C. Nowak, A. Neill & H. Liu - Structural analysis of variable domain glycosylation of anti-citrullinated protein antibodies in rheumatoid arthritis reveals the presence of highly sialylated glycans.
2016-12-12
Lise Hafkenscheid, Albert Bondt, Hans U. Scherer, Tom W.J. Huizinga, Manfred Wuhrer, René E.M. Toes and Yoann Rombouts - Custom-Designed Affinity Capture LC-MS F(ab')2 Assay for Biotransformation Assessment of Site-Specific Antibody Drug Conjugates
2016-10-25
D. Su, C. Ng, M. Khosraviani, SF. Yu, E. Cosino, S. Kaur and K. Xu - Quantitative twoplex glycan analysis using 12C6 and 13C6 stable isotope 2-aminobenzoic acid labelling and capillary electrophoresis mass spectrometry
2016-09-23
Csaba Váradi, Stefan Mittermayr, Silvia Millán-Martín and Jonathan Bones - Orthogonal Assessment of Biotherapeutic Glycosylation: A Case Study Correlating N-Glycan Core Afucosylation of Herceptin with Mechanism of Action
2016-09-12
Rosie Upton, Leonard Bell, Colin Guy, Paul Caldwell, Sian Estdale, Perdita E. Barran and David Firth - Target-independent variable region mediated effects on antibody clearance can be FcRn independent
2016-09-09
Ryan L. Kelly, Yao Yu, Tingwan Sun, Isabelle Caffry, Heather Lynaugh, Michael Brown, Tushar Jain, Yingda Xu & K. Dane Wittrup - Nonclinical Evaluation of PF-06438179: A Potential Biosimilar to Remicade® (Infliximab)
2016-09-01
Mazin Derzi, Theodore R. Johnson, Ahmed M. Shoieb, Hugh D. Conlon, Penny Sharpe, Andrew Saati, Sarah Koob, Michael W. Bolt, Leslie G. Lorello, Jim McNally, Carol F. Kirchhoff, Teresa A. Smolarek and Michael W. Leach - Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
2016-08-22
Jason J. Lohmueller, Shuji Sato, Lana Popova, Isabel M. Chu, Meghan A. Tucker, Roberto Barberena, Gregory M. Innocenti, Mare Cudic, James D. Ham, Wan Cheung Cheung, Roberto D. Polakiewicz, Olivera J. Finn - High-Throughput, Automated Protein A Purification Platform with Multiattribute LC-MS Analysis for Advanced Cell Culture Process Monitoring
2016-08-03
Jia Dong, Nicole Migliore, Steven J. Mehrman, John Cunningham, Michael J. Lewis and Ping Hu - Comparison of originator and biosimilar therapeutic monoclonal antibodies using comprehensive two-dimensional liquid chromatography coupled with time-of-flight mass spectrometry.
2016-06-30
Matthew Sorensen, David C. Harmes, Dwight R. Stoll, Gregory O. Staples, Szabolcs Fekete, Davy Guillarme & Alain Beck - Simple NMR methods for evaluating higher order structures of monoclonal antibody therapeutics with quinary structure
2016-06-07
Kang Chen Dianna S. Long Scott C. Lute Michaella J. Levy Kurt A. Brorson David A. Keire - A hingeless Fc fusion system for site-specific cleavage by IdeS
2016-05-21
Shabazz Novarra, Luba Grinberg, Keith W. Rickert, Arnita Barnes, Susan Wilson & Manuel Baca - Lipid- and polyion complex-based micelles as agonist platforms for TNFR superfamily receptors
2016-05-16
Ryan N. Gilbreth, Shabazz Novarra, Leslie Wetzel, Stelios Florinas, Horacio Cabral, Kazunori Kataoka, Jonathan RiosDoria, Ronald J. Christie, Manuel Baca - Rapid and improved characterization of therapeutic antibodies and antibody related products using IdeS digestion and subunit analysis
2016-05-04
Jonathan Sjögren, Fredrik Olsson and Alain Beck - Fc-fusion mimetics
2016-04-29
H. Khalili, P. T. Khaw and S. Brocchini - Characterization of cysteinylation and trisulfide bonds in a recombinant monoclonal antibody.
2016-04-26
Adriana Kita, Gomathinayagam Ponniah, Christine Nowak, and Hongcheng Liu - Potential of Hydrophilic Interaction Chromatography for the Analytical Characterization of Protein Biopharmaceuticals
2016-04-19
Aur´elie Periat Szabolcs Fekete Alessandra Cusumano Jean-Luc Veuthey Alain Beck Matthew Lauber Davy Guillarme - Characterization of the Co-elution of Host Cell Proteins with monoclonal antibodies during protein a purification
2016-04-16
Qingchun Zhang*, Andrew M. Goetze, Huanchun Cui, Jenna Wylie, Ben Tillotson, Art Hewig, Michael P. Hall and Gregory C. Flynn - Characterization of mAb dimers reveals predominant dimer forms common in therapeutic mAbs
2016-03-31
Friederike Plath, Philippe Ringler, Alexandra Graff-Meyer, Henning Stahlberg, Matthias E. Lauer, Arne C. Rufer, Melissa A. Graewert, Dmitri Svergun, Gerald Gellermann, Christof Finkler, Jan O. Stracke, Atanas Koulov & Volker Schnaible - Antigen-Specific Antibody Glycosylation Is Regulated via Vaccination.
2016-03-16
Mahan AE, Jennewein MF, Suscovich T, Dionne K, Tedesco J, Chung AW, Streeck H, Pau M, Schuitemaker H, Francis D, Fast P, Laufer D, Walker BD, Baden L, Barouch DH, Alter G. - Glycation of polyclonal IgGs: Effect of sugar excipients during stability studies
2016-03-15
Leblanc Y, Bihoreau N, Jube M, Andre MH, Tellier Z, Chevreux G - Structural characterization of antibody drug conjugate by a combination of intact, middle-up and bottom-up techniques using sheathless capillary electrophoresis – tandem mass spectrometry as nanoESI infusion platform and separation method
2016-03-06
Nassur Said, Rabah Gahoual, Lauriane Kuhn, Alain Beck, Yannis-Nicolas François, Emmanuelle Leize-Wagner - Characterization of Therapeutic Monoclonal Antibodies at the Subunit-Level using Middle-Down 193 nm Ultraviolet Photodissociation
2016-03-05
Victoria C. Cotham and Jennifer S. Brodbelt - Antibody-based PET imaging of amyloid beta in mouse models of Alzheimer’s disease
2016-02-19
Dag Sehlin, Xiaotian T. Fang, Linda Cato, Gunnar Antoni, Lars Lannfelt & Stina Syvänen - Full validation of therapeutic antibody sequences by middle-up mass measurements and middle-down protein sequencing
2016-01-13
Resemann A, Jabs W, Wiechmann A, Wagner E, Colas O, Evers W, Belau E, Vorwerg L, Evans C, Beck A, Suckau D - Characterization of Cetuximab Fc/2 subunits aggregates by off-line CZE-MS
2016-01-05
Yannis-Nicolas François, Michael Biacchi, Nassur Said, Charly Renard, Alain Beck, Rabah Gahoual, Emmanuelle Leize-Wagner - Intact mass analysis of monoclonal antibodies by capillary electrophoresis—Mass spectrometry
2015-12-20
Mei Han, Brooke M. Rock, Josh T. Pearson, Dan A. Rock - N-glycan composition profiling for quality testing of biotherapeutics
2015-12-01
Christopher Taron, Rebecca Duke - Comprehensive glycosylation profiling of IgG and IgG-fusion proteins by top-down MS with multiple fragmentation techniques
2015-10-15
Bao Quoc Tran, Christopher Barton, Jinhua Feng, Aimee Sandjong, Sung Hwan Yoon, Shivangi Awasthi, Tao Liang, Mohd M. Khan, David P.A. Kilgour, David R. Goodlett, Young Ah Goo - Determination of the NISTmAb Primary Structure
2015-10-15
Trina Formolo, Mellisa Ly, Michaella Levy, Lisa Kilpatrick, Scott Lute, Karen Phinney, Lisa Marzilli, Kurt Brorson, Michael Boyne, Darryl Davis, John Schiel - Pan-ebolavirus and pan-filovirus mouse monoclonal antibodies: protection against Ebola and Sudan viruses.
2015-10-14
Frederick W. Holtsberg, Sergey Shulenin, Hong Vu, Katie A. Howell, Sonal J. Patel, Bronwyn Gunn, Marcus Karim, Jonathan R. Lai, Julia C. Frei, Elisabeth K. Nyakatura, Larry Zeitlin, Robin Douglas, Marnie L. Fusco, Jeffrey W. Froude, Erica Ollmann Saphire, Andrew S. Herbert, Ariel S. Wirchnianski, Calli M. Lear-Rooney, Galit Alter, John M. Dye, Pamela J. Glass, Kelly L. Warfield & M. Javad Aman - Orthogonal Methods for Characterizing the Unfolding of Therapeutic Monoclonal Antibodies: Differential Scanning Calorimetry, Isothermal Chemical Denaturation, and Intrinsic Fluorescence with Concomitant Static Light Scattering
2015-10-09
Deniz B. Temel, Pavel Landsman, Mark L. Brader - Characterization of monoclonal antibodies by a fast and easy LC- MS ToF analysis on culture supernatant
2015-08-05
Antoine Henninot, Aurelie Terrier, Julie Charton, Rémi Urbain, Alexandre Fontayne, Benoit Deprez, Terence Beghyn - Characterization of the Acidic Species of a Monoclonal Antibody Using Weak Cation Exchange Chromatography and LC-MS
2015-07-29
Gomathinayagam Ponniah, Adriana Kita, Christine Nowak, Alyssa Neill, Yekaterina Kori, Saravanamoorthy Rajendran, Hongcheng Liu - Real-Time Product Attribute Control to Manufacture Antibodies with Defined N-Linked Glycan Levels
2015-07-14
Craig Zupke, Lowell J. Brady, Peter G. Slade, Philip Clark, R. Guy Caspary, Brittney Livingston, Lisa Taylor, Kyle Bigham, Arvia E. Morris, Robert W. Bailey - Direct Identification of Rituximab Main Isoforms and Subunit Analysis by Online Selective Comprehensive Two-Dimensional Liquid Chromatography−Mass Spectrometry
2015-07-06
Dwight R. Stoll, David C. Harmes, John Danforth, Elsa Wagner, Davy Guillarme, Szabolcs Fekete, Alain Beck - Analytical characterization of IgG Fc subclass variants through high-resolution separation combined with multiple LC-MS identification
2015-06-16
Xiaomei (Annie) He, Nathaniel Washburn, Enrique Arevalo, John H. Robblee - Design and Pharmacokinetic Characterization of Novel Antibody Formats for Ocular Therapeutics
2015-06-12
Kapil Gadkar, Cinthia V. Pastuskovas, Jennifer E. Le Couter, J. Michael Elliott, Jianhuan Zhang, Chingwei V. Lee, Sarah Sanowar, Germaine Fuh, Hok Seon Kim, T. Noelle Lombana, Christoph Spiess, Makia Nakamura, Phil Hass, Whitney Shatz, Y. Gloria Meng, Justin M. Scheer - Microscale purification of antigen-specific antibodies
2015-06-08
Eric P. Brown, Erica Normandin, Nana Yaw Osei-Owusu, Alison E. Mahan, Ying N. Chan, Jennifer I. Lai, Monica Vaccari, Mangala Rao, Genoveffa Franchini, Galit Alter, Margaret E. Ackerman - A Rapid Approach for Characterisation of Thiol-Conjugated ADCs and Calculation of Drug-Antibody Ratio by LC-MS
2015-06-01
David Firth, Leonard Bell, Martin Squires, Sian Estdale, Colin McKee - A chemical and computational approach to comprehensive glycation characterization on antibodies
2015-06-01
Ramsey A Saleem, Brittany R Affholter, Sihong Deng, Phil C Campbell, Kelli Matthies, Catherine M Eakin & Alison Wallace - Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 2: mass spectrometric methods
2015-05-21
Dietmar Reusch, Markus Haberger, David Falck, Britta Peter, Bernd Maier, Jana Gassner, Michaela Hook, Katharina Wagner, Lea Bonnington, Patrick Bulau, Manfred Wuhrer - The Impact II, a Very High-Resolution Quadrupole Time-of-Flight Instrument (QTOF) for Deep Shotgun Proteomics
2015-05-19
Scarlet Beck, Annette Michalski, Oliver Raether, Markus Lubeck, Stephanie Kaspar, Niels Goedecke, Carsten Baessmann, Daniel Hornburg, Florian Meier, Igor Paron, Nils A. Kulak, Juergen Cox, and Matthias Mann - Production of α2,6-sialylated IgG1 in CHO cells
2015-04-01
Céline Raymondab, Anna Robothama, Maureen Spearmanc, Michael Butlerc, John Kellya, Yves Durocher - Characterization of the N‐Terminal Heterogeneities of Monoclonal Antibodies Using In-Gel Charge Derivatization of α-Amines and LC- MS/MS
2015-03-13
Daniel Ayoub, Diego Bertaccini, Heĺeǹe Diemer, Elsa Wagner-Rousset, Olivier Colas, Sarah Cianfeŕani, Alain Van Dorsselaer, Alain Beck, Christine Schaeffer-Reiss - Sialylated IgG-Fc: a novel biomarker of chronic inflammatory demyelinating polyneuropathy
2015-03-02
Anna Hiu Yi Wong, Yuki Fukami, Makoto Sudo, Norito Kokubun, Shinsuke Hamada, Nobuhiro Yuki - Characterization of antibody drug conjugate positional isomers at cysteine residues by peptide mapping LC–MS analysis
2015-02-15
Marie-Claire Janin-Bussat, Marina Dillenbourg, Nathalie Corvaia, Alain Beck, Christine Klinguer-Hamour - A method for high-throughput, sensitive analysis of IgG Fc and Fab glycosylation by capillary electrophoresis
2015-02-10
Alison E. Mahan, Jacquelynne Tedesco, Kendall Dionne, Kavitha Baruah, Hao D. Cheng, Philip L. De Jager, Dan H. Barouch, Todd Suscovicha, Margaret Ackermanc, Max Cripsin, Galit Alter - Investigation of Antibody Disulfide Reduction and Re-oxidation and Impact to Biological Activities
2015-02-05
Tian Wang Yaoqing Diana Liu Bing Cai Gang Huang Gregory C. Flynn - Monocyte Recruitment by HLA IgG-Activated Endothelium: The Relationship Between IgG Subclass and FcγRIIa Polymorphisms
2015-02-03
N. M. Valenzuela, K. R. Trinh, A. Mulder, S. L. Morrison andE. F. Reed - Cutting-edge mass spectrometry characterization of originator, biosimilar and biobetter antibodies
2015-02-02
Alain Beck, François Debaene, Hélène Diemer, Elsa Wagner-Rousset, Olivier Colas, Alain Van Dorsselaer and Sarah Cianférani - Extensive glycosylation of ACPA-IgG variable domains modulates binding to citrullinated antigens in rheumatoid arthritis
2014-12-16
Yoann Rombouts, Annemiek Willemze, Joyce J B C van Beers, Jing Shi, Priscilla F Kerkman, Linda van Toorn, George M C Janssen, Arnaud Zaldumbide, Rob C Hoeben, Ger J M Pruijn, André M Deelder, Gertjan Wolbink, Theo Rispens, Peter A van Veelen, Tom W J Huizinga, Manfred Wuhrer, Leendert A Trouw, Hans U Scherer, René E M Toes - Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors
2014-12-15
Jérémy Loyaua, Pauline Malingea, Bruno Daubeufa, Limin Shanga, Greg Elsonab, Marie Kosco-Vilboisa, Nicolas Fischera & François Rousseaua - Structure and specificity of an antibody targeting a proteolytically cleaved IgG hinge
2014-08-01
Malia TJ, Teplyakov A, Brezski RJ, Luo J, Kinder M, Sweet RW, Almagro JC, Jordan RE, Gilliland GL - IgG Fab glycosylation analysis using a new mass spectrometric high-throughput profiling method reveals pregnancy-associated changes
2014-07-08
Bondt A, Rombouts Y, Selman MH, Hensbergen PJ, Reiding KR, Hazes JM, Dolhain RJ, Wuhrer M. - An EGFR Targeted PET Imaging Probe for the Detection of Colonic Adenocarcinomas in the Setting of Colitis
2014-07-01
Turker NS, Heidari P, Kucherlapati R, Kucherlapati M, Mahmood U - LC-MS analysis of polyclonal IgGs using IdeS enzymatic proteolysis for oxidation monitoring
2014-05-04
Leblanc Y, Romanin M, Bihoreau N, Chevreux G. - Double Blockade of CD14 and Complement C5 Abolishes the Cytokine Storm and Improves Morbidity and Survival in Polymicrobial Sepsis in Mice
2014-04-30
Huber-Lang M, Barratt-Due A, Pischke SE, Sandanger O, Nilsson PH, Nunn MA, Denk S, Gaus W, Espevik T, Mollnes TE. - Assessing analytical methods to monitor isoAsp formation in monoclonal antibodies
2014-04-29
Eakin CM, Miller A, Kerr J, Kung J, Wallace A. - A new tool for monoclonal antibody analysis Application of IdeS proteolysis in IgG domain-specific characterization
2014-04-07
Yan An, Ying Zhang, Hans-Martin Mueller, Mohammed Shameem, Xiaoyu Chen - From High- to Super-resolution Mass Spectrometry
2014-03-01
Tsybin YO - Middle-Down Analysis of Monoclonal Antibodies with Electron Transfer Dissociation Orbitrap Fourier Transform Mass Spectrometry
2014-02-18
Luca Fornelli, Daniel Ayoub, Konstantin Aizikov, Alain Beck, and Yury O. Tsybin - N- and O‑Glycosylation Analysis of Etanercept Using Liquid Chromatography and Quadrupole Time-of-Flight Mass Spectrometry Equipped with Electron-Transfer Dissociation Functionality
2013-12-05
Stephane Houel, Mark Hilliard, Ying Qing Yu, Niaobh McLoughlin, Silvia Millan Martin, Pauline M. Rudd, Jonathan P. Williams, and Weibin Chen - Antibody Drug Conjugate model fast characterization by LC-MS following IdeS proteolytic digestion.
2013-10-31
Wagner-Rousset E, Janin-Bussat MC, Colas O, Excoffier M, Haeuw JF, Rilatt I, Perez M, Corvaïa N, Beck A. - Rapid Fc glycosylation analysis of Fc fusions with IdeS and liquid chromatography mass spectrometry
2013-09-30
Lynaugh H, Li H, Gong B - Correct primary structure assessment and extensive glyco-profiling of cetuximab by a combination of intact, middle-up, middle-down and bottom-up ESI and MALDI mass spectrometry techniques.
2013-09-04
Ayoub D, Jabs W, Resemann A, Evers W, Evans C, Main L, Baessmann C, Wagner E, Suckau D, Beck A. - Profiling formulated monoclonal antibodies by (1)H NMR spectroscopy
2013-09-01
Poppe L, Jordan JB, Lawson K, Jerums M, Apostol I, Schnier PD. - Engineered protease-resistant antibodies with selectable cell-killing functions
2013-09-01
Kinder M, Greenplate AR, Grugan KD, Soring KL, Heeringa KA, McCarthy SG, Bannish G, Perpetua M, Lynch F, Jordan RE, Strohl WR, Brezski RJ - Formulation Development for Antibody-Drug Conjugates
2013-07-01
Junyan A. Ji, Jun Liu and Y. John Wang - Nondenaturing Size-Exclusion Chromatography-Mass Spectrometry to Measure Stress-Induced Aggregation in a Complex Mixture of Monoclonal Antibodies
2013-06-20
Woodard J, Lau H, Latypov RF - Analytical characterization of biosimilar antibodies and Fc- fusion proteins
2013-05-31
Beck A, Diemer H, Ayoub D, Debaene F, Wagner Rousset E, Carapito C, Dorsselaer Van A, Sanglier Cinférani S - Structural comparison of two anti-CD20 monoclonal antibody drug products using middle-down mass spectrometry
2013-04-12
Wang B, Gucinski AC, Keire DA, Buhse LF, Boyne Ii MT - Cetuximab Fab and Fc N-Glycan Fast Characterization Using IdeS Digestion and Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry.
2013-03-15
Janin-Bussat MC, Tonini L, Huillet C, Colas O, Klinguer-Hamour C, Corvaïa N, Beck A. - Engineering and Characterization of the Chimeric Antibody that Targets the C-terminal Telopeptide of the α2 Chain of Human Collagen I: A Next Step in the Quest to Reduce Localized Fibrosis.
2013-02-22
Fertala J, Steplewski A, Kostas J, Beredjiklian P, Williams G, Arnold W, Abboud J, Bhardwaj A, Hou C, Fertala A. - Fluorogenic Tagging Methodology Applied to Characterize Oxidized Tyrosine and Phenylalanine in an Immunoglobulin Monoclonal Antibody
2013-02-15
Shuxia Zhou, Olivier Mozziconacci, Bruce A. Kerwin & Christian Schöneich - Protein digestion: An overview of the available techniques and recent developments
2013-01-31
Linda Switzar, Martin Giera, and Wilfried M.A. Niessen - Glycoproteomic analysis of antibodies
2013-01-16
Gerhild Zauner, Maurice H. J. Selman, Albert Bondt, Yoann Rombouts, Dennis Blank, André M. Deelder, Manfred Wuhrer - Structural analysis of a therapeutic monoclonal antibody dimer by hydroxyl radical footprinting.
2013-01-03
Deperalta G, Alvarez M, Bechtel C, Dong K, McRonald R, Ling V - Characterization of Fc-Fusion Protein Aggregates Derived from Extracellular Domain Disulfide Bond Rearrangements
2012-12-14
James Strand, Chi-Ting Huang, Jon Xu - Characterization of therapeutic antibodies and related products
2012-11-07
Alain Beck , Elsa Wagner-Rousset , Daniel Ayoub , Alain Van Dorsselaer , and Sarah Sanglier-Cianférani - Proteomics-directed cloning of circulating antiviral human monoclonal antibodies
2012-11-01
Sato S, Beausoleil SA, Popova L, Beaudet JG, Ramenani RK, Zhang X, Wieler JS, Schieferl SM, Cheung WC, Polakiewicz RD - Characterization of critical reagents in ligand-binding assays: enabling robust bioanalytical methods and lifecycle management
2012-11-01
Geist BJ, Egan AC, Yang TY, Dong Y, Shankar G. - Comparative Binding of Disulfide-Bridged PEG-Fabs.
2012-10-18
Khalili H, Godwin A, Choi JW, Lever R, Brocchini S - Fc Gamma Receptor CD64 Modulates the Inhibitory Activity of Infliximab
2012-08-24
Kacper A. Wojtal1*, Gerhard Rogler1,2, Michael Scharl1, Luc Biedermann1, Pascal Frei, Michael Fried, Achim Weber, Jyrki J. Eloranta, Gerd A. Kullak-Ublick, Stephan R. Vavricka - Determination of antibody glycosylation by mass spectrometry.
2012-06-27
Jäger C, Ferrara C, Umaña P, Zeck A, Regula JT, Koll H - Quantitative glycan profiling of normal human plasma derived immunoglobulin and its fragments Fab and Fc.
2012-06-06
Anumula KR - Analysis and functional consequences of increased Fab-sialylation of intravenous immunoglobulin (IVIG) after lectin fractionation.
2012-06-04
Käsermann F, Boerema DJ, Rüegsegger M, Hofmann A, Wymann S, Zuercher AW, Miescher S - Sialylation-independent mechanism involved in the amelioration of murine immune thrombocytopenia using intravenous gammaglobulin
2012-01-18
Danila Leontyev, Yulia Katsman, Xue-Zhong Ma, Sylvia Miescher, Fabian Käsermann and Donald R. Branc - The in vitro resistance of IgG2 to proteolytic attack concurs with a comparative paucity of autoantibodies against peptide analogs of the IgG2 hinge.
2011-11-01
Brezski RJ, Oberholtzer A, Strake B, Jordan RE - Rapid LC-MS screening for IgG Fc modifications and allelic variants in blood
2011-09-25
Andrew M. Goetzea, Zhongqi Zhanga, Ling Liub, Frederick W. Jacobsenb, Gregory C. Flynn - Rates and impact of human antibody glycation in vivo.
2011-09-19
Goetze AM, Liu YD, Arroll T, Chu L, Flynn GC - Mechanism of immunoglobulin G4 Fab-arm exchange.
2011-06-15
Rispens T, Ooijevaar-de Heer P, Bende O, Aalberse RC - Fast analysis of recombinant monoclonal antibodies using IdeS proteolytic digestion and electrospray mass spectrometry.
2011-04-27
Chevreux G, Tilly N, Bihoreau N - Conjugation reactions in the preparations of quantum dot-based immunoluminescent probes for analysis of proteins by capillary electrophoresis.
2011-02-06
Lišková M, Voráčová I, Klepárník K, Hezinová V, Přikryl J, Foret F - Immunoglobulin cleavage by the streptococcal cysteine protease IdeS can be detected using protein G capture and mass spectrometry.
2007-05-05
Hess JL, Porsch EA, Shertz CA, Boyle MD