FabRICATOR MagIC

FabRICATOR® MagIC

FabRICATOR MagIC employs magnetic beads for fast automated processing of up to 96 antibodies in parallel.

The popular cysteine protease FabRICATOR (IdeS) immobilized on magnetic agarose beads delivers rapid and consistent subunit generation for multiple antibodies in parallel, perfect for fast automated analysis of CQAs in a middle-level LC/MS workflow.

Detailed Information

The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme digests IgG rapidly and specifically just below the hinge, generating F(ab’)2 and Fc fragments without any risk of over-digestion. The beads enable fast automated digestion of multiple antibodies in parallel in a middle-level characterisation workflow. They are designed for automation platforms like the Thermo Scientific™ KingFisher™ Duo Prime Purification System but perform equally on a shaking table if automation is not available. After digestion, the samples can be directly transferred to the autosampler of the LC-MS for fast chromatography or to another analytical method for analysis. Automated parallel antibody digestion and subunit generation allow for the processing of the numerous samples associated with clone selection, process development and stability studies for a potential biopharmaceutical. Automated antibody subunit analysis reduces operator time, risk for sample handling errors and increases the throughput.

Learn more about the applications of FabRICATOR MagIC

  • A0-FRM-096

    FabRICATOR MagIC 4 x 2 mL

    A0-FRM-0961,970.00Add to cart

Storage

FabRICATOR MagIC is supplied as a 25% bulk resin in vials. The product is shipped cold and should be stored at +4-8°C.

Performance  

The performance of FabRICATOR MagIC at 37°C is typically less than 20 minute digestion with efficiency of >95% for 20-200 µg human IgG1 (1 mg/ml) sample in PBS, pH 7.4.

Key Characteristics

  • Automated workflow for middle level analysis
  • Compatible with automation platforms
  • High throughput parallel analysis of antibodies
  • Complete digestion in 10-20 minutes

Product Overview

Product size: 2 ml and 4 x 2 ml
Sample size: Digestion of up to 24 x 200 µg IgG (2ml) and 96 x 200 µg IgG (4 x 2 ml)
Short Specification: 20µL settled magnetic agarose resin digest >=95% of 20 and 200µg human IgG1 in 100µL 10 mM Sodium Phosphate, 150 mM NaCl pH 7.4 in 20 minutes at 37°C as monitored by SDS-PAGE
Starting material: Human IgG1-4, Fc-fusion proteins
Operating temperature: 37°C
Storage conditions: +4°-8°C
Bead material: Magnetic agarose beads
Compatible Additives: 5mM TECP for generation of reduced subunits, Fd’, LC and scFc.
0.05% Polysorbate 20 in the digestion buffer to minimize shear stress in automated systems.

 

  • Human IgG1-4, some classes of IgG from monkey, rat, rabbit and sheep
  • 96 samples in less than 20 minutes
  • Over 95% digestion of 20-200 µg IgG

Documents

Instructions

Product Specifications

Safety Data Sheet

Certificate of Analysis

How to Order

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Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

FAQ and Support

Popular FAQ

FabRICATOR MagIC is suitable for using in automated systems that can handle magnetic beads. On the applications page you can read about how it’s used in a KingFisher™ workflow.

The protocol for FabRICATOR MagIC is based on using standard PBS pH 7.4 as digestion buffer. FabRICATOR in solution is compatible with most commonly used buffers at pH=6-8 and the same buffers should work for FabRICATOR MagIC, but the incubation time might need to be optimized.

The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.

FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).

In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2.

 

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