FabRICATOR-HPLC

FabRICATOR® MagIC

FabRICATOR MagIC employs magnetic beads for fast automated processing of up to 96 antibodies in parallel.

The popular cysteine protease FabRICATOR (IdeS) immobilized on magnetic agarose beads delivers rapid and consistent subunit generation for multiple antibodies in parallel, perfect for fast automated analysis of CQAs in a middle-level LC/MS workflow.

Detailed Information

The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme digests IgG rapidly and specifically just below the hinge, generating F(ab’)2 and Fc fragments without any risk of over-digestion. The beads enable fast automated digestion of multiple antibodies in parallel in a middle-level characterisation workflow. They are designed for automation platforms like the Thermo Scientific™ KingFisher™ Duo Prime Purification System but perform equally on a shaking table if automation is not available. After digestion, the samples can be directly transferred to the autosampler of the LC-MS for fast chromatography or to another analytical method for analysis. Automated parallel antibody digestion and subunit generation allow for the processing of the numerous samples associated with clone selection, process development and stability studies for a potential biopharmaceutical. Automated antibody subunit analysis reduces operator time, risk for sample handling errors and increases the throughput.

Learn more about the applications of FabRICATOR MagIC

  • A0-FRM-096

    FabRICATOR MagIC 4 x 2 mL

    A0-FRM-0961,875.00Add to cart

Storage

FabRICATOR MagIC is supplied as a 25% bulk resin in vials. The product is shipped cold and should be stored at +4-8°C.

Performance  

The performance of FabRICATOR MagIC at 37°C is typically less than 20 minute digestion with efficiency of >95% for 20-200 µg human IgG1 (1 mg/ml) sample in PBS, pH 7.4.

Key Characteristics

  • Automated workflow for middle level analysis
  • Compatible with automation platforms
  • High throughput parallel analysis of antibodies
  • Complete digestion in 10-20 minutes

Product Overview

Product size: 2 ml and 4 x 2 ml
Sample size: Digestion of up to 24 x 200 µg IgG (2ml) and 96 x 200 µg IgG (4 x 2 ml)
Short Specification: 20µL settled magnetic agarose resin digest >=95% of 20 and 200µg human IgG1 in 100µL 10 mM Sodium Phosphate, 150 mM NaCl pH 7.4 in 20 minutes at 37°C as monitored by SDS-PAGE
Starting material: Human IgG1-4, Fc-fusion proteins
Operating temperature: 37°C
Storage conditions: +4°-8°C
Bead material: Magnetic agarose beads
Compatible Additives: 5mM TECP for generation of reduced subunits, Fd’, LC and scFc.
0.05% Polysorbate 20 in the digestion buffer to minimize shear stress in automated systems.

 

  • Human IgG1-4, some classes of IgG from monkey, rat, rabbit and sheep
  • 96 samples in less than 20 minutes
  • Over 95% digestion of 20-200 µg IgG

Documents

Instructions

Product Specifications

Safety Data Sheet

Certificate of Analysis

How to Order

For details on ordering please visit How to order. Click add to cart above to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities or licensing opportunities.

FAQ and Support

Popular FAQ

We have tested PBS and 150 mM ammonium acetate, pH7 with good results. More buffers were found to be compatible with FabRICATOR digestion in solution (more info) and should be suitable for FabRICATOR MagIC as well.

The FabRICATOR MagIC magnetic beads with immobilized FabRICATOR (IdeS) enzyme.

Yes, it is possible to digest the antibody using an automation system. See more in the applications page.

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Legal and Disclaimers

FabRICATOR®

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.For more information about commercial rights, please contact Genovis team at info@genovis.com. Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Thermo Scientific™, KingFisher™ and BindIt™

The trademarks Thermo Scientific™, KingFisher™ and BindIt™ are the property of Thermo Fisher Scientific Inc. and its subsidiaries.

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