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FragIT™ Z 

The FragIT Z spin columns contain FabRICATOR Z (IdeZ) enzyme covalently coupled to agarose beads, for digestion of mouse IgG2a and IgG3 without contaminating the final preparation with enzyme.

FabRICATOR Z is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc fragments after 2 hours incubation.

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Detailed Information

FragIT Z consists of FabRICATOR Z enzyme immobilized on agarose beads in a convenient spin column format. The spin columns are available for digestion of up to 0.5 mg (Microspin) mouse IgG2a and IgG3 in three different pack sizes, with 2, 5 or 10 columns per pack.

FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, producing a homogenous pool of F(ab')2 and Fc fragments. Most mouse IgG2a are digested by FabRICATOR Z within 2 hours although the enzyme:ab ratio and/or digestion time may need to be optimized for certain antibodies. There is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR Z digests IgG under physiological reaction conditions, the immunoreactivity is preserved.

Storage and Stability

FragIT Z is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!

Unit Definition

One spin column contains sufficient FabRICATOR Z-coupled agarose beads to digest 0.5 mg mouse IgG2a or IgG3.

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How to Order

For details on ordering please visit How to order. Click add to cart to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

Instructions

Safety Data Sheet

FAQ and Support

Latest FAQ

FabRICATOR® cleave the IgG molecule in one specific site in the lower hinge region of the anitbody leaving a F(ab')2 fragment and a Fc-fragment.

Human IgG1: HTCPPCPAPELLG⬇GPSVF
Human IgG2: VECPPCPAPP_VA⬇GPSVF
Human IgG3: PPCPRCPAPELLG⬇GPSVF
Human IgG4: PHAHHAQAPEFLG⬇GPSVF

You can indeed elute the Fc fragments from the CaptureSelect column using a low pH buffer commonly used for example during protein A/G purification of antibodies. We have successfully used 0.1 M glycine buffer pH 2.6-2.8 to release the Fc fragments. Be sure to adjust pH back to normal using a 1 M Tris-HCl buffer or similar directly after isolation of the Fc fragments from the CaptureSelect column.

To use protein A or MabSelect Sure (GE) for removal of Fc as a platform after digestion with FabRICATOR or FragIT is problematic. Therefore we have chosen CaptureSelect in the FragIT Kit for removal of Fc fragments. CaptureSelect is much more specific for Fc. Both protein A and MabSelect Sure (GE) may have affinity for Fab and the degree of affinity is antibody dependent. We have many customer reports on this subject and they usually comes back to us for the CaptureSelect columns for the above reason.

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Patent and Disclaimer


FabRICATOR®


Genovis AB hold a license for exclusive worldwide use of biotechnical industrial applications of IdeS covered by any patents derived from International publication WO03051914, including granted US Patent No US7666582.
Other aspects of FabRICATOR®technology are encompassed by pending patent applications in the name of Genovis AB.

The trademark FabRICATOR® is the property of Genovis AB.


For research use only. Not intended for any animal or human therapeutic diagnostic use.