FragIT™ Z kit
FragIT Z kit is used for the preparation and purification of F(ab')2 and Fc fragments from mouse IgG2a and IgG3. The kit consists of spin columns of FragIT Z (Immobilized FabRICATOR Z) for antibody digestion and spin columns of CaptureSelect™* Fc resin for affinity purification of the Fc fragment.
FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc fragments after 2 hours incubation.
Detailed Information
FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, producing a homogenous pool of F(ab')2 and Fc fragments. Most mouse IgG2a are digested by FabRICATOR Z within 2 hours although the enzyme:ab ratio and/or digestion time may need to be optimized for certain antibodies. There is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR Z digests IgG under physiological reaction conditions, the immunoreactivity is preserved.
FragIT Z is a resin with the FabRICATOR Z (IdeZ) enzyme covalently coupled to agarose beads for subunit fragmentation of mouse IgG2a and IgG3 without contaminating the final preparation with enzyme. The FragIT Z kit consists of FragIT Z resin and CaptureSelect™ columns containing multispecies Fc affinity matrix. The used ligand is directed towards the Fc domain of IgG enabling purification of IgG from several species, including human, mouse, bovine, rabbit, rat, goat, horse and sheep.
The FragIT Z kit is available for digestion of 0.5 mg or 5 x 0.5 mg mouse IgG2a or IgG3.
Storage and Stability
The FragIT Z spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!
Buffers
FragIT Z can be used with all commonly used buffers with pH ranging from 6.0 to 8.0. Optimization may be required. Note that the FabRICATOR Z enzyme is irreversibly inactivated below pH 5.0.
Unit Definition
One spin column contains sufficient FabRICATOR Z-coupled agarose beads to digest 0.5 mg mouse IgG2a or IgG3.
*Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
FragIT Z kit, Digest and Purify up to 0.5 mg
A2-FZ2-005€400.00Add to cart
FragIT Z kit, Digest and Purify up to 5 x 0.5 mg
A2-FZ2-025€1,070.00Add to cart
How to Order
For details on ordering please visit How to order. Click add to cart to buy online.
Quotations
Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.
Documents
Instructions
Product Specifications
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
FabRICATOR® cleave the IgG molecule in one specific site in the lower hinge region of the anitbody leaving a F(ab’)2 fragment and a Fc-fragment.
Human IgG1: HTCPPCPAPELLG / GPSVF
Human IgG2: VECPPCPAPP_VA / GPSVF
Human IgG3: PPCPRCPAPELLG / GPSVF
Human IgG4: PHAHHAQAPEFLG / GPSVF
Genovis has chosen CaptureSelect in the FragIT Kit for removal of Fc fragments. CaptureSelect has a very high specific affinity for the Fc. Both protein A and MabSelect Sure (GE) may have affinity for the Fab which may result in a lower recovery of the generated F(ab’)2 fragments and the degree of affinity is antibody dependent. We have many customer reports on this subject, and they usually come back to us for the CaptureSelect columns for the above reason.
We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.
FabRICATOR is a cysteine protease. It should not result in any other modifications than the digestion of the peptide bond. Digestion will be between the two Glycines (…CPAPELLG / GPSVF…) and the C-terminus will regain the carboxyl group – that is addition of one OH-group and the N-terminal amino group will be regained (addition of one H).
In summary, R1-CO-NH-R2 + H2O –> R1-COOH + NH2-R2.
Load More
Legal and Disclaimers
All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.
For more information about commercial rights, please contact Genovis team at info@genovis.com.
Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.