FragIT Z kit

FragIT Z kit

FragIT Z kit is used for the preparation and purification of F(ab')2 and Fc fragments from mouse IgG2a and IgG3. The kit consists of spin columns of FragIT Z (Immobilized FabRICATOR Z) for antibody digestion and spin columns of CaptureSelect™* Fc resin for affinity purification of the Fc fragment. 

FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc fragments after 2 hours incubation.

Detailed Information

FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, producing a homogenous pool of F(ab')2 and Fc fragments. Most mouse IgG2a are digested by FabRICATOR Z within 2 hours although the enzyme:ab ratio and/or digestion time may need to be optimized for certain antibodies. There is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR Z digests IgG under physiological reaction conditions, the immunoreactivity is preserved.

FragIT Z is a resin with the FabRICATOR Z (IdeZ) enzyme covalently coupled to agarose beads for subunit fragmentation of mouse IgG2a and IgG3 without contaminating the final preparation with enzyme. The FragIT Z kit consists of FragIT Z resin and CaptureSelect™ columns containing multispecies Fc affinity matrix. The used ligand is directed towards the Fc domain of IgG enabling purification of IgG from several species, including human, mouse, bovine, rabbit, rat, goat, horse and sheep.

The FragIT Z kit is available for digestion of 0.5 mg or 5 x 0.5 mg mouse IgG2a or IgG3.

Storage and Stability

The FragIT Z spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!

Buffers

FragIT Z can be used with all commonly used buffers with pH ranging from 6.0 to 8.0. Optimization may be required. Note that the FabRICATOR Z enzyme is irreversibly inactivated below pH 5.0.

Unit Definition

One spin column contains sufficient FabRICATOR Z-coupled agarose beads to digest 0.5 mg mouse IgG2a or IgG3.

 

*Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

FragIT-Z-kit-flow

  • A2-FZ2-005

    FragIT Z kit, Digest and Purify up to 0.5 mg

    A2-FZ2-005440.00Add to cart
  • A2-FZ2-025

    FragIT Z kit, Digest and Purify up to 5 x 0.5 mg

    A2-FZ2-0251,170.00Add to cart

How to Order

For details on ordering please visit How to order. Click add to cart to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

Instructions

Safety Data Sheet

Certificate of Analysis

FAQ and Support

Popular FAQ

We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.

It is possible to elute the Fc fragments from the CaptureSelect column using a low pH buffer, for example a 0.1M Glycine buffer pH=3.0. The pH must immediately after elution be adjusted to neutral pH by adding 0.1x elution volume of 1M Tris pH=8.0.

 

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