FabRICATOR® Z Enzyme
FabRICATOR Z Enzyme is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for digestion of up to 2 mg mouse IgG2a and IgG3.
FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc fragments after 2 hours incubation.
Detailed Information
FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, producing a homogenous pool of F(ab')2 and Fc fragments. Most mouse IgG2a are digested by FabRICATOR Z within 2 hours although the enzyme:ab ratio and/or digestion time may need to be optimized for certain antibodies. There is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR Z digests IgG under physiological reaction conditions, the immunoreactivity is preserved.
FabRICATOR Z is derived from S. equi ssp. zooepidemicus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 36 442 Da.
Storage and Stability
The FabRICATOR Z enzyme is supplied lyophilized, and is reconstituted by the addition of water. After reconstitution, FabRICATOR Z is stable for 1 month at +4-8°C. The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
Unit Definition
One unit digests ≥ 95% of 1µg mouse IgG2a when incubated in 10 mM Tris, 10 mM NaCl, pH 6.5 at 37°C for 2 hours.
FabRICATOR Z, 2000 Units
A0-FRZ-020€470.00Add to cart
How to Order
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Quotations
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Documents
Instructions
Product Specification
Safety Data Sheet
Certificate of Analysis
FAQ and Support
Popular FAQ
Yes. FabRICATOR is compatible with EDTA. Make sure your pH is within 6-8.
If we assume that the antibody concentration in serum is 15 mg/ml you would have to add 10-15 000 units to a sample of 1 ml serum. In 50 µl serum you would have to add 500 – 750 units of FabRICATOR.
Yes, FabRICATOR works on hamster IgG using the standard protocol as for human IgG.
No. According to the following reference, FabRICATOR does not digest IgM. Pavel-Rammingen et al., 2002. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G.
We have tested 4-6 M GuHCl which the enzyme does not withstand without significant decrease of activity. SDS have not been evaluated but 0.1% RapiGest from waters have negative influence on activity. Taken together the enzyme does not well tolerate denaturing agents although we have not tested this in more detailed studies.
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Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.