FabRICATOR®

FabRICATOR®Z Antibody Cleavage
product-box-fabricatorz

FabRICATOR® Z (IdeZ)

FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc fragments after 2 hours incubation.

 

FabRICATOR Z is available in three different product formats:

Product Information

FabRICATOR Z is an enzyme for preparation of F(ab’)2 from mouse subclasses IgG2a and IgG3. FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, generating intact F(ab’)2 and Fc fragments. Most mouse IgG2a and IgG3 are digested by FabRICATOR Z within 2 hours, although the enzyme:antibody ratio and/or digestion time may need to be optimized for certain antibodies. For digestion of human IgGs, FabRICATOR® is recommended.

 

FabRICATOR Z digests IgG at a specific site and there is no risk of overdigestion if the incubation time is prolonged. FabRICATOR Z digests IgG at mild conditions, thus preserving the immunoreactivity. FabRICATOR Z digests IgG in sodium phosphate buffer with pH ranging from 6.0-8.0, but is most active at pH 6.5 and at low NaCl concentrations.

 

FabRICATOR Z is derived from S. equi ssp. zooepidemicus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 36 442 Da.

Key Characteristics

  • Mouse IgG2a and IgG3, IgG from monkey, rabbit and sheep
  • 2 h reaction
  • No need for reducing agents or co-factors
  • CPAPNLLG / GPSVF (below the hinge)

Applications

  • Digest mouse IgG2a and IgG3

Product Formats

FabRICATOR® Z Enzyme

The FabRICATOR Z enzyme supplied as a lyophilized powder. The enzyme is available in 2000 unit vials for digestion of up to 2 mg mouse IgG2a or IgG3. 

FragIT™ Z (Immobilized FabRICATOR Z)

FragIT Z is FabRICATOR Z (IdeZ) enzyme immobilized on agarose beads, allowing for mouse IgG2a and IgG3 digestion without contaminating the final preparation with FabRICATOR Z enzyme.

FragIT™ Z kit

FragIT Z Kit easily generates and purifies F(ab')2 and Fc/2 fragments from mouse IgG2a and IgG3. The kit consists of two parts; FragIT Z spin columns and affinity spin columns for purification of F(ab'2) fragments. 

Download

Brochures and Posters

Download Genovis brochures and posters with the latest details on the SmartEnzymes

Smart Enzyme Reference Tool

SmartEnzyme Reference Tool

Find the right reference for the antibody attribute you are studying

Applications

Applications

Learn more about the applications of Genovis SmartEnzymes

FAQ and Support

FAQ

There are not yet any FAQ's for this product. If you have a technical question, please submit a support case. 

Here you can view all our FAQ's

Patent and Disclaimer


FabRICATOR®

Genovis AB hold a license for exclusive worldwide use of biotechnical industrial applications of IdeS covered by any patents derived from International publication WO03051914, including granted US Patent No US7666582.
Other aspects of FabRICATOR technology are encompassed by pending patent applications in the name of Genovis AB.

The trademark FabRICATOR is the property of Genovis AB.


For research use only. Not intended for any animal or human therapeutic diagnostic use.