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FabRICATOR®Z IdeZ Antibody Cleavage

FabRICATOR® Z (IdeZ)

FabRICATOR Z (IdeZ) is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge, generating a homogenous pool of F(ab')2 and Fc fragments after 2 hours incubation.

FabRICATOR Z is available in three different product formats:

Product Information

FabRICATOR Z is an enzyme for preparation of F(ab’)2 from mouse subclasses IgG2a and IgG3. FabRICATOR Z digests mouse IgG2a and IgG3 at a specific site below the hinge region, generating intact F(ab’)2 and Fc fragments. Most mouse IgG2a and IgG3 are digested by FabRICATOR Z within 2 hours, although the enzyme:antibody ratio and/or digestion time may need to be optimized for certain antibodies. For digestion of human IgGs, FabRICATOR® is recommended.

 

FabRICATOR Z digests IgG at a specific site and there is no risk of overdigestion if the incubation time is prolonged. FabRICATOR Z digests IgG at mild conditions, thus preserving the immunoreactivity. FabRICATOR Z digests IgG in sodium phosphate buffer with pH ranging from 6.0-8.0, but is most active at pH 6.5 and at low NaCl concentrations.

 

FabRICATOR Z is derived from S. equi ssp. zooepidemicus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 36 442 Da.

Key Characteristics

  • Mouse IgG2a and IgG3, IgG from monkey, rabbit and sheep
  • 2 h reaction
  • No need for reducing agents or co-factors
  • CPAPNLLG / GPSVF (below the hinge)

Applications

  • Digest mouse IgG2a and IgG3

Product Formats

FabRICATOR® Z Enzyme

The FabRICATOR Z enzyme supplied as a lyophilized powder. The enzyme is available in 2000 unit vials for digestion of up to 2 mg mouse IgG2a or IgG3. 

FragIT™ Z (Immobilized FabRICATOR Z)

FragIT Z is FabRICATOR Z (IdeZ) enzyme immobilized on agarose beads, allowing for mouse IgG2a and IgG3 digestion without contaminating the final preparation with FabRICATOR Z enzyme.

FragIT™ Z kit

FragIT Z Kit easily generates and purifies F(ab')2 and Fc/2 fragments from mouse IgG2a and IgG3. The kit consists of two parts; FragIT Z spin columns and affinity spin columns for purification of F(ab'2) fragments. 

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FabRICATOR-Z Applications

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All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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