Detailed Information
FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge, generating intact Fab and Fc fragments. The name of the enzyme is derived from the pathogen Streptococcus agalactiae, where it was first discovered. The enzyme is active at pH from 6-8 and does not require reducing conditions or co-factors for activity.
FabALACTICA is recombinantly expressed in E. coli, contains a His-tag, and the molecular weight is 70 019 Da.
Storage and Stability
The Immobilized FabALACTICA spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!
Unit Definition
One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.
*Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
