Genovis / Products / IgG Proteases / FabALACTICA / FabALACTICA Fab kit

FabALACTICA Fab kit

FabALACTICA® Fab kit

FabALACTICA Fab kit is used for the preparation and purification of Fab fragments from human IgG1. The kit consists of spin columns of Immobilized FabALACTICA for antibody digestion and spin columns of CaptureSelect™* Fc resin for purification of the Fab fragments.

FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at one specific site above the hinge with no need for reducing conditions or co-factors.

 

Detailed Information

FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge, generating intact Fab and Fc fragments. The name of the enzyme is derived from the pathogen Streptococcus agalactiae, where it was first discovered. The enzyme is active at pH from 6-8 and does not require reducing conditions or co-factors for activity.

FabALACTICA is recombinantly expressed in E. coli, contains a His-tag, and the molecular weight is 70 019 Da.

Storage and Stability

The Immobilized FabALACTICA spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!

Unit Definition

One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.

 

*Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

FabALACTICA-Fab-kit

  • A2-AFK-005

    FabALACTICA Fab kit Microspin, Digest and purify up to 0.5 mg hIgG1

    A2-AFK-005€440.00Add to cart
  • A2-AFK-025

    FabALACTICA Fab kit Microspin, Digest and purify up to 5 x 0.5 mg hIgG1

    A2-AFK-025€1,160.00Add to cart
  • A2-AFK-100

    FabALACTICA Fab kit Midispin, Digest and purify up to 10 mg hIgG1

    A2-AFK-100€1,430.00Add to cart
  • A2-AFK-1000

    FabALACTICA Fab kit Maxispin, Digest and purify up to 100 mg hIgG1

    A2-AFK-1000€4,350.00Add to cart

How to Order

For details on ordering please visit How to order. Click add to cart to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

FAQ and Support

Popular FAQ

b

Can I release Fc fragments from the CaptureSelect column after isolating the Fab fragments?

It is not recommended to elute the Fc fragments from the CaptureSelect column since the fraction might also include undigested or semi digested material.

b

Can I re-use immobilized FabALACTICA columns?

We can only guarantee optimal digestion for one-time use. We do not have a cleaning or regeneration protocol to provide. However, depending on the antibody and the following application the column can be reused. We do not recommend using with different antibodies due to the risk for contamination of carry-over from previous sample. If column reuse is desired store the column in 10-20% ethanol at +4-8°C.

 

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