FabALACTICA Fab kit

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FabALACTICA® Fab kit

FabALACTICA Fab kit is used for the preparation and purification of Fab fragments from human IgG1. The kit consists of spin columns of Immobilized FabALACTICA for antibody digestion and spin columns of CaptureSelect™* Fc resin for purification of the Fab fragments.

FabALACTICA (IgdE) is a cysteine protease that digests human IgG1 at one specific site above the hinge with no need for reducing conditions or co-factors.

 

Detailed Information

FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge, generating intact Fab and Fc fragments. The name of the enzyme is derived from the pathogen Streptococcus agalactiae, where it was first discovered. The enzyme is active at pH from 6-8 and does not require reducing conditions or co-factors for activity.

FabALACTICA is recombinantly expressed in E. coli, contains a His-tag, and the molecular weight is 70 019 Da.

Storage and Stability

The Immobilized FabALACTICA spin columns and CaptureSelect™* spin columns are supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the products!

Unit Definition

One spin column contains sufficient FabALACTICA-coupled agarose beads to digest 0.5 mg (Microspin), 10 mg (Midispin), or 100 mg (Maxispin) human IgG1.

 

*Thermo Scientific™ CaptureSelect™ resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.

FabALACTICA-Fab-kit

How to Order

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Quotations

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Documents

FAQ and Support

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Patent and Disclaimer

FabALACTICA®

Aspects of FabALACTICA® technology are encompassed by pending patent applications in the name of Genovis AB. The trademark FabALACTICA® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.