Genovis / Products / IgG Proteases

Enzymes for O-glycans

Enzymes for O-glycans

IgG Proteases

The Genovis product line of IgG proteases consists of:

FabRICATOR - a cysteine protease digesting IgG at a specific site below the hinge

FabALACTICA - a cysteine protease digesting human IgG1 at a specific site above the hinge

GingisKHAN - a cysteine protease digesting human IgG1 at a specific site above the hinge

FabULOUS - a cysteine protease digesting IgG in the hinge region

FabRICATOR Z - a cysteine protease digesting mouse IgG2a at a specific site below the hinge

 

An overview of the enzymes is presented on this page. Please visit the individual product pages for full details on the different IgG proteases.

 

Overview

Our proteases digest IgG at different sites. An overview of the digestion sites in human IgG1 is found in the picture to the right.

 

FabRICATOR (IdeS) is a cysteine protease that digests IgG and Fc-fusion proteins at a specific site below the hinge region, yielding one F(ab’)2 fragment and two Fc fragments.

 

Key Characteristics

  • Human IgG1-4, IgG from monkey, rat, rabbit and sheep
  • 30 min reaction
  • No need for reducing agents or co-factors
  • CPAPELLG / GPSVF (below the hinge)

FabALACTICA is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact Fab and Fc fragments. No reducing agents or co-factors are required for enzymatic activity.

 

Key Characteristics

  • Human IgG1
  • Overnight (O/N) reaction
  • No need for reducing agents or co-factors
  • KSCDKT / HTCPPCP (above the hinge)

GingisKHAN is a cysteine protease that digests human IgG1 at a specific site above the hinge, generating intact Fab and Fc fragments. 

 

Key Characteristics

  • Human IgG1
  • 60 min reaction
  • 2 mM cysteine (included)
  • KSCDK / THTCPPCP (above the hinge)

FabULOUS (SpeB) is a cysteine protease that digests IgG in the hinge region of antibodies from many species and subclasses, including human, mouse, rat and goat.

 

Key Characteristics

  • Human IgG1-4, IgG from mouse, rat, goat, sheep and rabbit
  • 60 min reaction
  • Requires reducing conditions
  • Human IgG1: KTHT / CPPCPAP (above the hinge)

FabRICATOR Z is a cysteine protease that digests mouse IgG2a and IgG3 at a specific site below the hinge. 

 

Key Characteristics

  • Mouse IgG2a and IgG3, IgG from monkey, rabbit and sheep
  • 2 h reaction
  • No need for reducing agents or co-factors
  • CPAPNLLG / GPSVF (below the hinge)

Patent and Disclaimer

FabRICATOR®, FabALACTICA®, GingisKHAN®, FabULOUS® and FabRICATOR® Z

All rights reserved. The trademarks FabRICATOR®, FabALACTICA®, GingisKHAN®, FabULOUS® and FabRICATOR® Z are the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.