Enzymes for O-glycans

Enzymes for O-glycans

IgG Proteases

The Genovis product line of IgG proteases consists of:

FabRICATOR - a cysteine protease digesting IgG at one specific site below the hinge

FabALACTICA - a cysteine protease digesting human IgG1 at one specific site above the hinge

GingisKHAN - a cysteine protease digesting human IgG1 at one specific site above the hinge

FabULOUS - a cysteine protease digesting IgG in the upper hinge region

FabRICATOR Z - a cysteine protease digesting mouse IgG2a at one specific site below the hinge

 

An overview of the enzymes is presented on this page. Please visit the individual product pages for full details on the different IgG proteases.

 

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Overview

Our proteases digest IgG at different sites. An overview of the digestion sites in human IgG1 is found in the picture to the right.

 

FabRICATOR (IdeS) is a cysteine protease that digests IgG and Fc-fusion proteins at one specific site below the hinge region, yielding one F(ab’)2 fragment and two Fc fragments. 

 

Key Characteristics

  • Specific - one precise digestion site below the hinge
  • Rapid digestion - 30 minute protocol
  • No optimization needed, and no risk of overdigestion
  • Excellent yields - close to 100%
  • Robust - works every time

FabALACTICA is a cysteine protease that digests human IgG1 at one specific site above the hinge, generating intact Fab and Fc fragments.

 

Key Characteristics

  • High specificity for digestion above the hinge of human IgG1
  • No need for reducing agents or co-factors
  • No risk of overdigestion

GingisKHAN is a cysteine protease that digests human IgG1 at one specific site above the hinge. 

 

Key Characteristics

  • Specific - one precise digestion site above the hinge of human IgG1
  • Generates intact Fab fragments
  • Requires mild reducing conditions for optimal activity
  • Rapid - 60 minutes digestion protocol
  • Provided with ready-to-use reaction buffer additive, for convenient and reliable performance

FabULOUS (SpeB) is a cysteine protease that digests IgG in the upper hinge region of antibodies from many species and subclasses, including human, mouse, rat and goat.

 

Key Characteristics

  • Digestion of IgG in the upper hinge region under reducing conditions
  • Active on several IgG species, including human, mouse, rat and goat
  • Rapid protocol – 1 hour digestion

FabRICATOR Z is a cysteine protease that digests mouse IgG2a at one specific site below the hinge. 

 

Key Characteristics

  • Enhanced and rapid digestion of mouse IgG2a below the hinge
  • Rapid generation of F(ab')2 and Fc/2 fragments
  • No risk of overdigestion

Patent and Disclaimer

FabRICATOR®, FabALACTICA™, GingisKHAN®, FabULOUS® and FabRICATOR® Z

All rights reserved. The trademarks FabRICATOR®, FabALACTICA™, GingisKHAN®, FabULOUS® and FabRICATOR® Z are the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2017 Genovis AB.