Immobilized GlycINATOR

product-box-glycinator

Immobilized GlycINATOR®

The Immobilized GlycINATOR spin columns contain GlycINATOR enzyme covalently coupled to agarose beads, for deglycosylation of Fc-glycans without contaminating the final preparation with enzyme.

GlycINATOR (EndoS2) is an IgG-specific endoglycosidase that hydrolyzes all glycoforms present at the Fc-glycosylation site. The enzyme acts on the chitobiose core and leaves the core GlcNAc.

 

 

Detailed Information 

Immobilized GlycINATOR enzyme on agarose in convenient spin columns. The spin columns are available in three different sizes for deglycosylation of up to 0.5 mg (MicroSpin), 10 mg (MidiSpin) or 100 mg (MaxiSpin) IgG. The smallest columns are available in three different pack sizes, with 2, 5, or 10 columns per pack.

Immobilized GlycINATOR hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc intact on the Fc. IgG is incubated with the agarose beads for 15 min and deglycosylated IgG is then collected by a 1 minute centrifugation step. 

Immobilized GlycINATOR deglycosylates IgG under physiological reaction conditions, thus preserving the immunoreactivity. Deglycosylation is performed at room temperature. GlycINATOR deglycosylates all human IgG subclasses and IgG from many different species, including mouse, rat, monkey, sheep, goat, cow, and horse.

Storage and stability

Immobilized GlycINATOR® is supplied in 20% ethanol, shipped on ice, and should be stored at +4-8°C upon arrival. Do not freeze the product!

Unit definition

One spin column contains sufficient GlycINATOR-coupled agarose beads to deglycosylate 0.5 mg (MicroSpin), 10 mg (MidiSpin), or 100 mg (MaxiSpin) IgG.

 

How to order

To place an order, please visit How to order. Click add to cart to buy online.

Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries on price of bulk quantities.

Documents

FAQ and Support

FAQ

There are not yet any FAQ's for this product. If you have a technical question, please submit a support case.

Here you can view all our FAQ's

Patent and Disclaimer

GlycINATOR®

The trademark GlycINATOR is the property of Genovis AB.


For research use only. Not intended for any animal or human therapeutic or diagnostic use.