GlycINATOR LE (Low Endotoxin) enzyme is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for deglycosylation of up to 2 mg IgG. This product formulation has low levels of endotoxins per vial (< 0.2 EU).
GlycINATOR (EndoS2) is an IgG-specific endoglycosidase that hydrolyzes all glycoforms present at the Fc-glycosylation site. The enzyme acts on the chitobiose core and leaves the core GlcNAc.
GlycINATOR LE hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc intact on the Fc. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 92 kDa. GlycINATOR deglycosylates all human IgG subclasses and IgG from many different species, including mouse, rat, monkey, sheep, goat, cow, and horse.
The GlycINATOR LE enzyme is supplied lyophilized and reconstituted by addition of water. After reconstitution, GlycINATOR LE is stable for 1 month at +4-8°C. The product is shipped at ambient temperature but should be stored at -20°C upon arrival.
One unit deglycosylates ≥ 95% of 1μg human IgG when incubated in 10mM sodium phosphate, 150mM NaCl, pH 7.4 at 37°C for 30min.
Patent and Disclaimer
The trademark GlycINATOR is the property of Genovis AB.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.