SmartEnzymes FucosEXO workflow

Hydrolysis of α1-2,3,4 Fucose

FucosEXO is a mix of α-Fucosidases for efficient hydrolysis of α1-2, α1-3 and α1-4-linked fucose residues on native N- and O-glycosylated proteins or free oligosaccharides.

It is a valuable tool for glycan structure analysis on N- and O-glycoproteins and in exoglycosidase arrays on oligosaccharides.
 

  • Highly efficient α-Fucosidase mixture – fast removal of α1-2, α1-3 and α1-4-linked fucose
  • Reliable digestion increases confidence in exoglycosidase arrays
  • Available immobilized in ready-to-use spin columns
Fucose on N- and O-glycosylated proteins
Hydrolyzes ɑ1-2,3,4-linked fucose
1-2 h reaction
No need for co-factors

FucosEXO Lyophilized

Lyophilized enzyme for defucosylation of glycoproteins

FucosEXO Immobilized

Immobilized enzyme for defucosylation of glycoproteins in spin columns

About FucosEXO™

FucosEXO workflow

FucosEXO is a mix of α-Fucosidases for efficient hydrolysis of α1-2, α1-3 and α1-4-linked fucose on N- and O-glycosylated proteins or free oligosaccharides.

The enzymes in FucosEXO are expressed in E. coli with a His-tag, and have molecular weights of 87 kDa and 64 kDa. FucosEXO hydrolyzes fucose on glycoproteins under native conditions and displays high activity in a pH range from 6 to 8, without the need for co-factors or additives.

FucosEXO™ Lyophilized

Lyophilized enzyme for defucosylation of glycoproteins.

FucosEXO Lyophilized is available as a lyophilized powder in 2000 unit vials for defucosylation of 2 mg glycoprotein.
 

  • Flexible format for method development
  • Possible to combine enzymes for improved efficiency
  • Amenable to automated workflows
  • Ready to use – just add water!

Available Products

FucosEXO Lyophilized 2000 unitsLyophilized enzyme for defucosylation of 2 mg glycoproteinG1-FM1-020904.00Buy / Request a Quote

For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

Unit Definition

One unit of FucosEXO Lyophilized hydrolyzes fucoses from ≥90% of 11 nmol 2'-fucosyllactose and 4.5 nmol 3-fucosyllactose when incubated in 20 mM Tris pH 6.8 at 37°C for 30 min.

Content and Storage

FucosEXO Lyophilized is reconstituted by addition of water. No preservatives added. After reconstitution, FucosEXO Lyophilized is stable for 1 month at +4-8°C. The product is shipped cold and should be stored at -20 °C upon arrival.

Instructions

Product Specification

Safety Data Sheet

Certificate of Analysis

FucosEXO™ Immobilized

Immobilized enzyme for defucosylation of glycoproteins in spin columns.

The FucosEXO Immobilized spin columns contain FucosEXO enzyme covalently coupled to agarose beads, for defucosylation of glycoprotein without contaminating the final preparation with enzyme.

The Microspin columns are available for defucosylation of 5 or 10 x 0.5 mg glycoprotein.

 

  • Easy-to-use spin columns
  • Increased enzyme-to-substrate ratio for improved digestion efficiency
  • No enzyme in the final sample

Available Products

FucosEXO Immobilized Microspin 5 x 0.5 mgImmobilized enzyme for defucosylation of 5 x 0.5 mg glycoprotein in spin columnsG1-FM6-025959.00Buy / Request a Quote
FucosEXO Immobilized Microspin 10 x 0.5 mgImmobilized enzyme for defucosylation of 10 x 0.5 mg glycoprotein in spin columnsG1-FM6-0501,566.00Buy / Request a Quote

For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

Unit Definition

One Microspin column contains sufficient FucosEXO-coupled agarose beads to defucosylate 0.5 mg glycoprotein.

Content and Storage

FucosEXO Immobilized is supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!

Instructions

Product Specification

Safety Data Sheet

Certificate of Analysis

FAQ and Support

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