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Detailed Information - GingisREX^®

GingisREX is a cysteine protease that specifically digests peptide bonds C-terminally to arginine residues, including arginine linked to proline. Cysteine is required for enzymatic activity.

The enzyme is purified from Porphyromonas gingivalis.

Stability and Storage

The enzyme is provided as a lyophilized powder. After reconstitution by addition of water, GingisREX is stable for at least 1 month at 4-8°C. The product is shipped cold and should be stored at -20°C upon arrival.

Buffers and Reducing Agent

To maintain the enzymatically active cysteine in a reduced form, TCEP is added to preserve the reducing environment (for details please consult the user instructions). GingisREX is active in a broad pH range, 5.0-9.0, with optimal activity between pH 6.5-8.0. Digestion can be performed in the presence of chaotropic agents or detergents such as urea, SDS, tween and SDC. Buffers tested and compatible with GingisREX activity are Tris, Bis-Tris and ammonium bicarbonate.

FAQ and Support

Popular FAQ

Is cysteine really needed for activation of the cysteine protease if TCEP or DTT is added in the reaction?

Yes, cysteine is required for both activity and specificity of the GingisREX enzyme.

Is the reaction ready in only 60 minutes?

The digestion time is affected by many factors like the enzyme to sample ratio, pH of the digestion reaction, and the sample characteristics. The reaction might need to be optimized for each specific case. In general, we recommend starting with an overnight digestion.

Can guanidinium hydrochloride be used as a denaturing agent instead of urea?

No, GingisREX shows significantly reduced activity in the presence of Gd-HCl even in low concentrations. This chaotrope should be avoided.

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GingisREX^®