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GingisKHAN® Enzyme

GingisKHAN enzyme is a unique protease that digests human IgG1 between K223 and T224 producing a homogenous pool of Fab and Fc fragments. The enzyme requires only mild reduced condition for activity and the reaction is performed in the presence of 2 mM Cystein at neutral pH, leaving intact Fab and Fc fragments.

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Products

GingisKHAN enzyme is supplied as lyophilized preparation together with 5 vials of lyophilized GingisKHAN reducing agent for convenient and reliable performance. The product is available in 2000 unit format for digestion of up to 2 mg human IgG1.

Quotation & Ordering

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Please find information for placing orders at How to Order

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Detailed Information

Digestion of human IgG1 with GingisKHAN generates a homogenous pool of precise Fab and Fc fragments. There is no over-digestion or further degradation of the fragments typically associated with other proteolytic enzymes. Very mild reducing reaction conditions give intact Fab and Fc fragments. Best activity is obtained at 37°C.

GingisKHAN is shipped as a lyophilized preparation together with 5 vials of lyophilized GingisKHAN reducing agent for convenient and reliable performance.

Storage

GingisKHAN is supplied as lyophilized powder. It is supplied with 5 vials of lyophilized GingisKHAN reducing agent (10x) containing 20 mM cysteine upon reconstitution.

GingisKHAN and GingisKHAN Reducing Agent are shipped on ice and should be stored at -20°C upon arrival.

After reconstitution GingisKHAN is stable for at least 1 month +4-8°C. Reconstituted GingisKHAN Reducing Agent cannot be stored, use freshly prepared.

Unit definition

One unit cleaves ≥ 95% of 1μg human IgG1 when incubated in 0.1 M Tris, pH 8.0 at 37°C for 1 hour.

Documents

Applications

Production of Fab Fragments

Intact Fab fragments from human IgG1 can be generated in 1 hour using GingisKHAN (KGP) enzyme.

For other IgG subclasses and species there is another procedure using a combination of our FabRICATOR enzyme with 2-MEA (2-mercaptoethylamine or Cysteamine). This procedure is simple and usually gives good yields. It can be completed in two hours.

Find all applications here

Enzyme Reference Tool

All published papers on how Genovis enzymes help simplify antibody analytics. With this tool you can filter by quality attribute, enzyme used, type of antibody molecule and technology.

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Brochures and Posters

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FAQ and Support

FAQ

There are not yet any FAQ's for this product. If you have a technical question, please submit a support case. 

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Patent and Disclaimer


GingisKHAN®

The trademark GingisKHAN is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic diagnostic use.