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FabULOUS Fab kit

Use FabULOUS Fab kit to generate purified and homogenous Fab fragments. The kit involves digestion of mouse IgG with FabULOUS and purification of the Fab fragments on LC-Kappa (murine) specific affinity spin columns.

Products

FabULOUS Fab kit contains one vial of FabULOUS enzyme with 2000 units for digestion of up to 2 mg mouse IgG and 4 LC-Kappa (mur) micro spin columns where each column includes sufficient material to purify up to 0.5 mg IgG.

Quotation & ordering

Don't hesitate to contact us if you would like to receive a quotation.
Please find information for placing orders at How to Order.

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Detailed Information

FabULOUS Fab kit is for rapid and easy preparation and purification of Fab fragments from mouse antibodies. The kit contains FabULOUS enzyme for digestion of mouse IgG and LC-Kappa specific affinity spin columns for purification of the Fab fragments.

FabULOUS enzyme is a unique recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region producing a homogenous pool of Fab fragments. The enzyme requires reduced condition for activity and reaction is performed in the presence of 50mM Cysteine at neutral pH.

The CaptureSelect LC-Kappa (mur)* affinity matrix recognizes the LC-Kappa domain of murine IgG antibodies, which enables purification of Fab fragments.

Storage

FabULOUS Fab kit is shipped on ice.

FabULOUS 2000u should be stored at -20°C upon arrival.

CaptureSelect LC-Kappa (mur) resin* in MicroSpin columns should be stored at +4-8°C.

After reconstitution of FabULOUS enzyme, it is stable for 1 months at +4-8°C.

Unit definition

One unit digests ≥ 95% of 1µg human or mouse IgG when incubated in 10 mM Tris, 150 mM NaCl pH 7.4 at 37°C for 1 hour.

Applications

Purified and homogenous Fab fragments can be used in many applications, including:

  • Study monovalent binding of Fab fragments to the antigen
  • Elimination of Fc-mediated effector functions
  • Reduced unspecific binding from Fc interactions 

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Figure 1. A) Intact monoclonal mouse IgG1 antibody. B) Analysis of fragments after FabULOUS Fab kit digestion. C) Eluted Fab fragments from the CaptureSelectTM LC-Kappa column. Digestion and puri cation were analyzed on RP-HPLC (Agilent 1290) using Waters Acquity UPLC® BEH300 C4.

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Figure 2. Non-reducing SDS-PAGE analysis of puri ed Fab fragments from monoclonal mIgG1 using FabULOUS Fab kit. Lane 1;intact mIgG1, Lane 2; digested mIgG1, Lane 3; flow through from CaptureSelect column, Lane 4; eluted Fab fragments, Lane 5; MW marker.

Documents

Instructions

Product specifications

Enzyme Reference Tool

All published papers on how Genovis enzymes help simplify antibody analytics. With this tool you can filter by quality attribute, enzyme used, type of antibody molecule and technology.

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FAQ and Support

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Patent and Disclaimer


FabULOUS®

The trademark FabULOUS® is the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic diagnostic use.
*Made with Thermo Scientific CaptureSelect resin from Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Scientific and CaptureSelect are trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.