FabULOUS is a recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region.
The enzyme requires reduced condition for activity and reaction is performed in the presence of 1-5 mM DTT or TCEP at neutral pH.
- Digestion of IgG in the hinge region
- Active on IgG from many species, e.g. human, mouse, rat and goat
- Rapid protocol – 1 hour digestion
FabULOUS® Primary Digestion Site
Human IgG1 ..KTHT / CPPAPAPA..
Mouse IgG1 ..KPCIC / TVPEVS..
For more species please visit Applications to view the IgG protease digestion chart for more species and subclasses
FabULOUS enzyme supplied as lyophilized powder. The enzyme is available in a 2000 unit vial (1 unit digest 1 ug IgG).
FabULOUS Fab kit mouse is used for rapid and easy preparation and purification of Fab fragments from mouse IgG. The kit consists of two parts; lyophilized FabULOUS enzyme and affinity spin columns for purification of intact Fab fragments.
Documents and References
Patent and Disclaimer
All rights reserved. Aspects of FabULOUS technology are encompassed by pending patent applications in the name of Genovis AB.
The trademark FabULOUS is the property of Genovis AB. FabULOUS is duly registered in EU, China, Japan, Australia, Canada, South Korea and USA.
For research use only. Not intended for any animal or human therapeutic diagnostic use.
All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
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