FabULOUS® (SpeB)

FabULOUS is a recombinantly produced cysteine protease that digests antibodies from many species and subclasses in the hinge region.

The enzyme requires reduced condition for activity and reaction is performed in the presence of 1-5 mM DTT or TCEP at neutral pH.

Key Characteristics

  • Digestion of IgG in the hinge region
  • Active on IgG from many species, e.g. human, mouse, rat and goat
  • Rapid protocol – 1 hour digestion

FabULOUS® Primary Digestion Site


Mouse IgG1 ..KPCIC / TVPEVS..

For more species please visit Applications to view the IgG protease digestion chart for more species and subclasses

Product Formats

FabULOUS® Enzyme

FabULOUS enzyme supplied as lyophilized powder. The enzyme is available in a 2000 unit vial (1 unit digest 1 ug IgG).

FabULOUS® Fab kit mouse

FabULOUS Fab kit mouse is used for rapid and easy preparation and purification of Fab fragments from mouse IgG. The kit consists of two parts; lyophilized FabULOUS enzyme and affinity spin columns for purification of intact Fab fragments.

Documents and References

Product Documents


Enzyme Reference Tool

All published papers on how Genovis enzymes help simplify antibody analytics

FAQ and Support


We have not found that FabULOUS (SpeB) digest human IgG4. For other subclasses and species tested please visit

Find all FAQ here

Patent and Disclaimer


All rights reserved. Aspects of FabULOUS technology are encompassed by pending patent applications in the name of Genovis AB.
The trademark FabULOUS is the property of Genovis AB. FabULOUS is duly registered in EU, China, Japan, Australia, Canada, South Korea and USA.
For research use only. Not intended for any animal or human therapeutic diagnostic use.
All goods and services are sold subject to Genovis’ General Terms and Conditions of Sale.
©2016 Genovis AB.