SialEXO is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for desialylation of up to 2 mg glycoprotein.

It is a sialidase mix for complete removal of sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins or released glycans.

Detailed Information

SialEXO is a mixture of two sialidases for efficient desialylation of O- and N-glycosylated proteins. The sialidases in the mixture have unique activities and work simultaneously. One enzyme has a broad activity for α2-3, α2-6 and α2-8 linked sialic acids and the other one quickly hydrolyzes sialic acids with α2-3 linkage. Removes all sialic acids on native proteins within 2 h.

Hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 6.5 to 9.

The enzymes are derived from Akkermansia muciniphila and expressed in E. coli. Both enzymes contain a His-tag and the molecular weight of each enzyme is 43 kDa and 66 kDa, respectively.

Unit Definition

One unit of SialEXO hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

Supplied lyophilized in TBS  pH 7.6, with no preservatives added.
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
After reconstitution SialEXO is stable for at least 1 month at +4-8 °C.

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Product Specifications

Safety Data Sheet

Certificate of Analysis

FAQ and Support

Popular FAQ

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.

Yes, SialEXO contains a 6x His-tag.

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.

Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.


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