SialEXO

SialEXO®

SialEXO is supplied as a lyophilized powder with no preservatives added, and is available in 2000 unit vials for desialylation of up to 2 mg glycoprotein.

It is a sialidase mix for complete removal of sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins or released glycans.

Detailed Information

SialEXO is a mixture of two sialidases for efficient desialylation of O- and N-glycosylated proteins. The sialidases in the mixture have unique activities and work simultaneously. One enzyme has a broad activity for α2-3, α2-6 and α2-8 linked sialic acids and the other one quickly hydrolyzes sialic acids with α2-3 linkage. Removes all sialic acids on native proteins within 2 h.

Hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 6.5 to 9.

The enzymes are derived from Akkermansia muciniphila and expressed in E. coli. Both enzymes contain a His-tag and the molecular weight of each enzyme is 43 kDa and 66 kDa, respectively.

Unit Definition

One unit of SialEXO hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.

Content and Storage

Supplied lyophilized in TBS  pH 7.6, with no preservatives added.
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
After reconstitution SialEXO is stable for at least 1 month at +4-8 °C.

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Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries regarding price for bulk quantities or licensing opportunities.

Documents

Instructions

Product Specifications

Safety Data Sheet

Certificate of Analysis

FAQ and Support

Popular FAQ

Yes, SialEXO contains a 6x His-tag.

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.

Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.

Yes, both enzymes are active under neutral conditions, preferably use 20 mM Tris pH 7.

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.

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Legal and Disclaimers

All rights reserved. Genovis products are covered by one or more patents, pending patent applications, trademarks and/or copyrights owned or controlled by Genovis AB.

For more information about commercial rights, please contact Genovis team at info@genovis.com.

Genovis products are intended for research use only. They are not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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