SialEXO 23

SialEXO® 23

SialEXO 23 is supplied as a lyophilized powder with no preservatives added, and is available in 500 unit vials for desialylation of up to 0.5 mg glycoprotein.

SialEXO 23 is an α2-3 specific sialidase.

Detailed Information

In contrast to SialEXO which is a sialidase mix, SialEXO 23 is an α2-3 specific sialidase, allowing targeted analysis of α2-3 linked sialic acids only. Efficient α2-3 desialylation of O- and N-glycosylated proteins can be achieved within 1 h.

SialEXO 23 hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 7.0 to 9.0.

The enzyme is derived from Akkermansia muciniphila and expressed in E. coli. The enzyme has a His-tag and the molecular weight is 66 kDa.

Unit Definition

One unit of SialEXO 23 hydrolyzes α2-3 linked sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 7.5 at 37 °C for 1 h.

Content and Storage

SialEXO 23 is supplied lyophilized in TBS pH 7.6, with no preservatives added.
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
After reconstitution SialEXO 23 is stable for at least 1 month at +4-8 °C.

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Product Specifications

Safety Data Sheet

Certificate of Analysis

FAQ and Support

Popular FAQ

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.

Yes, SialEXO contains a 6x His-tag.

Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.


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