Detailed Information

In contrast to SialEXO which is a sialidase mix, SialEXO 23 is an α2-3 specific sialidase, allowing targeted analysis of α2-3 linked sialic acids only. Efficient α2-3 desialylation of O- and N-glycosylated proteins can be achieved within 1 h.

SialEXO 23 hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 7.0 to 9.0.

The enzyme is derived from Akkermansia muciniphila and expressed in E. coli. The enzyme has a His-tag and the molecular weight is 66 kDa.

Unit Definition

One unit of SialEXO 23 hydrolyzes α2-3 linked sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 7.5 at 37 °C for 1 h.

Content and Storage

SialEXO 23 is supplied lyophilized in TBS pH 7.6, with no preservatives added.
The product is shipped at ambient temperature, and should be stored at -20°C upon arrival.
After reconstitution SialEXO 23 is stable for at least 1 month at +4-8 °C.