Immobilized SialEXO

Immobilized SialEXO®

The Immobilized SialEXO Microspin columns contain SialEXO covalently coupled to agarose beads, for desialylation of up to 0.5 mg native glycoproteins in 30 minutes without any residual enzyme in the final preparation.

Hydrolyzes sialic acids (α2-3, α2-6 and α2-8) on native glycoproteins or released glycans.

Detailed Information

Immobilized SialEXO is a resin with a mixture of two sialidases covalently coupled to agarose beads for complete removal of sialic acids (α2-3, α2-6 & α2-8, linked) on O- and N-glycosylated proteins. The enzymes in SialEXO are derived from Akkermansia muciniphila and expressed in E. coli.

Desialylated proteins are generated without any residual enzyme in the final preparation. The glycoprotein sample is incubated with the resin for 30 minutes at room temperature and the desialylated glycoproteins are then easily collected by a centrifugation step. The recommended buffer is 20 mM Tris pH 6.8. The protocol may need optimization regarding buffer compatibility and incubation time for individual glycoproteins.

Storage and Stability

Supplied in 20% ethanol, shipped cold, and should be stored at +4-8°C upon arrival. Do not freeze the product!

Unit Definition

0.1 ml of settled Immobilized SialEXO agarose resin hydrolyzes sialic acids from > 90% of 0.5 mg C1 inhibitor in 30 min at room temperature in PBS pH 7.4 as monitored by ion exchange chromatography.

  • G1-SM6-010

    Immobilized SialEXO Microspin 2 x 0.5 mg

    G1-SM6-010380.00Add to cart
  • G1-SM6-025

    Immobilized SialEXO Microspin, 5 x 0.5 mg

    G1-SM6-025810.00Add to cart
  • G1-SM6-050

    Immobilized SialEXO Microspin, 10 x 0.5 mg

    G1-SM6-0501,350.00Add to cart

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Quotations

Please do not hesitate to contact us if you need a quotation or have inquiries regarding price for bulk quantities or licensing opportunities.

Documents

Product Specifications

Safety Data Sheet

Certificate of Analysis

Download Immobilized SialEXO poster

Immobilized SialEXO Brochure

Download the Immobilized SialEXO brochure

Applications Immobilized SialEXO

Applications of Immobilized SialEXO

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Immobilized Sialexo poster 1

Immobilized SialEXO Poster

"Complete and Rapid Desialylation of Therapeutic Glycoproteins"

FAQ and Support

Popular FAQ

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.

Yes, SialEXO contains a 6x His-tag.

Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.

 

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