SialEXO®
The SialEXO family are sialidase products for the removal and analysis of sialic acids. The enzymes are active on N- or O-linked glycans present on native glycoproteins or released glycan structures.
SialEXO is available in three different product formats:
- SialEXO - sialidase mix for complete removal of sialic acids (α2-3, α2-6 & α2-8)
- SialEXO 23 - an α2-3 specific sialidase
- Immobilized SialEXO - immobilized SialEXO on agarose beads
Product Information
The SialEXO family are sialidase products for the removal and analysis of sialic acids on native glycoproteins or on released glycans. The sialidases have unique activities and they work fast with no need for co-factors. They are active on both N- and O-linked glycans.
Product Formats
SialEXO®
SialEXO for complete removal of sialic acids (α2-3, α2-6 and α2-8). It is supplied as a lyophilized powder. SialEXO is available in 2000 unit vials for desialylation of up to 2 mg glycoprotein.
SialEXO® 23
SialEXO 23 for complete removal of α2-3 sialic acids. It is supplied as a lyophilized powder. The enzyme is available in 500 unit vials for desialylation of up to 0.5 mg glycoprotein.
Immobilized SialEXO®
SialEXO immobilized on agarose beads, allowing for desialylation of 0.5 mg glycoprotein in 30 minutes without enzyme in the final preparation.
Key Characteristics
- Hydrolyzes sialic acids on N- and O-linked glycans
- α2-3, α2-6 and α2-8-linked sialic acids
- 30 min - 2 h reaction
- Requires no co-factors
Applications
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Brochures, Posters and Applications
Brochures and Posters
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External References and Reference Tool
FAQ and Support
Popular FAQ
Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.
Yes, SialEXO contains a 6x His-tag.
Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.
No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.
Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.
