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FucosEXO workflow

Efficient hydrolysis of α1-2,3,4 linked fucose

FucosEXO is an α-fucosidase mix for efficient removal of α1-2, α1-3 and α1-4 linked fucose from N- and O-glycoproteins or free oligosaccharides. FucosEXO is active in neutral pH and does not require any additives or co-factors.

FucosEXO is available in two different product formats:

FucosEXO Enzyme - Lyophilized α-fucosidase mix for defucosylation of 2 mg of glycoprotein.

Immobilized FucosEXO - Immobilized α-fucosidase mix in microspin columns for defucosylation of 0.5 mg of glycoprotein.

FucosEXO Enzyme

FucosEXO is a mix of α-fucosidases for efficient hydrolysis of α1-2, α1-3 and α1-4-linked fucose on N- and O-glycosylated proteins or free oligosaccharides. The enzymes in FucosEXO are expressed in  E. coli with a His-tag, and have molecular weights of 87 kDa and 64 kDa. FucosEXO hydrolyzes fucose on glycoproteins under native conditions and displays high activity in a pH range from 6 to 8, without the need for co-factors or additives. The enzyme is provided lyophilized in 2000 unit vials or immobilized on agarose beads in a micro spin column format for processing of up to 0.5 mg of glycoprotein without enzyme in the final preparation.

 

Unit Definition

One unit of FucosEXO hydrolyzes fucoses from ≥90% of 11 nmol 2'-fucosyllactose and 4.5 nmol 3-fucosyllactose when incubated in 20 mM Tris pH 6.8 at 37°C for 30 min.

 

Content and Storage

FucosEXO is supplied lyophilized in TBS pH 7.6, with no preservatives added. FucosEXO is shipped cold and should be stored at -20 °C upon arrival. After reconstitution FucosEXO is stable for at least 1 month at +4-8 °C.

Immobilized FucosEXO Microspin columns contain sufficient material each to remove fucoses from 0.5 mg glycoprotein. The resin is supplied in 20% EtOH with no preservatives added. Immobilized FucosEXO is shipped cold and should be stored at +4-8°C upon arrival. Do not freeze the product!

FucosEXO and Immobilized FucosEXO is for R&D use only.

  • G1-FM6-025

    Immobilized FucosEXO Microspin, Digest 5 x 0.5 mg

    G1-FM6-025795.00Add to cart
  • G1-FM6-050

    Immobilized FucosEXO Microspin, Digest 10 x 0.5 mg

    G1-FM6-0501,295.00Add to cart

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FucosEXO Enzyme Brochure

FucosEXO Brochure

FucosEXO hydrolyzes α1-2,3,4 linked fucose on glycoproteins.

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