Enzymes for O-glycans

Enzymes for O-glycans

Enzymes for O-glycan Analysis

The Genovis product line of enzymes for O-glycans consists of:

OpeRATOR® – O-protease for O-glycan-specific digestion of glycoproteins

GlycOCATCH™ – Tool for enrichment of O-glycosylated proteins and peptides

OglyZOR® – Endoglycosidase (O-glycosidase) for O-glycans of core 1 and core 3

SialEXO® – Exoglycosidase acting on sialic acids

An overview of the enzymes are presented on this page. Please visit the individual product pages for full details on the different enzymes for O-glycans.



OpeRATOR is an O-glycan-specific protease that digests O-glycosylated proteins N-terminally of Ser and Thr glycosylation sites.


Key Characteristics

  • Digests native O-glycosylated proteins
  • 2h to overnight (16-18h) reaction
  • Desialylation using SialEXO® (included) increases performance
  • N-terminally of O-glycosylated serine and threonine residues


GlycOCATCH is an enrichment resin for affinity purification of O-glycosylated proteins and peptides.


Key Characteristics

  • Binds O-glycosylated proteins and peptides
  • 2h binding
  • Desialylation using SialEXO® (included) increases binding
  • Glycoproteins and peptides carrying O-glycans


OglyZOR is an O-glycosidase that specifically hydrolyzes core 1 and core 3 type O-glycans on native glycoproteins.


Key Characteristics

  • Hydrolyzes O-glycans on glycoproteins
  • 2-4h reaction
  • Requires removal of sialic acids using SialEXO® (included)
  • Core 1 and core 3 type O-glycan disaccharides


SialEXO is a sialidase mix for complete removal of sialic acids from 2 mg native glycoprotein.


Key Characteristics

  • Hydrolyzes sialic acids on N- and O-linked glycans
  • 2 h reaction
  • Requires no co-factors
  • α2-3, α2-6 and α2-8-linked sialic acids





Patent and Disclaimer

OpeRATOR®, GlycOCATCH™, OglyZOR® and SialEXO®

All rights reserved. Aspects of OpeRATOR®, GlycOCATCH™, OglyZOR®,  and SialEXO® technologies are encompassed by pending patent applications in the name of Genovis AB.

The trademarks OpeRATOR®, GlycOCATCH™, OglyZOR®, and SialEXO® are the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.