Enzymes for O-glycans

Enzymes for O-glycans

Enzymes for O-glycan Analysis

The Genovis product line of enzymes for O-glycans consists of:

OpeRATOR™ - O-protease for O-glycan-specific digestion of glycoproteins

GlycOCATCH™ - Tool for enrichment of O-glycosylated proteins and peptides

OglyZOR™ - Endoglycosidase (O-glycosidase) for O-glycans of core 1 and core 3

SialEXO™ - Exoglycosidase acting on sialic acids

An overview of the enzymes are presented on this page. Please visit the individual product pages for full details on the different enzymes for O-glycans.

 

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OpeRATOR is an O-glycan-specific protease that digests O-glycosylated proteins N-terminally of Ser and Thr glycosylation sites.

 

Key Characteristics

  • Digests native O-glycosylated proteins
  • 2h to overnight (16-18h) reaction
  • Desialylation using SialEXO™ (included) increases performance
  • N-terminally of O-glycosylated serine and threonine residues

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GlycOCATCH is an enrichment resin for affinity purification of O-glycosylated proteins and peptides.

 

Key Characteristics

  • Binds O-glycosylated proteins and peptides
  • 2h binding
  • Desialylation using SialEXO™ (included) increases binding
  • Glycoproteins and peptides carrying O-glycans

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OglyZOR is an O-glycosidase that specifically hydrolyzes core 1 and core 3 type O-glycans on native glycoproteins.

 

Key Characteristics

  • Hydrolyzes O-glycans on glycoproteins
  • 2-4h reaction
  • Requires removal of sialic acids using SialEXO™ (included)
  • Core 1 and core 3 type O-glycan disaccharides

product-logo-sialexo

SialEXO is a sialidase mix for complete removal of sialic acids from 2 mg native glycoprotein.

 

Key Characteristics

  • Hydrolyzes sialic acids on N- and O-linked glycans
  • 2 h reaction
  • Requires no co-factors
  • α2-3, α2-6 and α2-8-linked sialic acids

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Patent and Disclaimer

OpeRATOR™, GlycOCATCH™, OglyZOR™ and SialEXO™

All rights reserved. Aspects of OpeRATOR™, GlycOCATCH™, OglyZOR™,  and SialEXO™ technologies are encompassed by pending patent applications in the name of Genovis AB.

The trademarks OpeRATOR™, GlycOCATCH™, OglyZOR™, and SialEXO™ are the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.