Enzymes for O-glycans

Enzymes for O-glycans

Enzymes for O-glycan Analysis

The Genovis product line of enzymes for O-glycans consists of:

OpeRATOR™ - O-protease for O-glycan-specific digestion of glycoproteins

GlycOCATCH™ - Tool for enrichment of O-glycosylated proteins and peptides

OglyZOR™ - Endoglycosidase for O-glycans (disaccharides) of core 1 and core 3

SialEXO™ - Exoglycosidase acting on sialic acids

An overview of the enzymes are presented on this page. Please visit the individual product pages for full details on the different enzymes for O-glycans.

 

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OpeRATOR is an O-protease digesting O-glycosylated proteins N-terminally of the S/T glycosylation site.

Key Characteristics

  • O-glycan specific endoprotease
  • Digests N-terminally of O-glycan-occupied serine or threonine
  • Requires O-glycan for activity
  • No activity on glycoproteins with only N-glycans
  • Pretreatment with SialEXO™ increases performance

 

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GlycOCATCH is an enrichment resin for specific purification of O-glycosylated proteins and peptides.

Key Characteristics

  • Enriches O-glycosylated proteins and peptides
  • 2 h binding
  • Requires removal of sialic acids using SialEXO (included)
  • Elution is performed using 8 M urea or OpeRATOR (included)
  • Applications in glycomics and biopharmaceutical characterization

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OglyZOR is an endoglycosidase that specifically hydrolyzes core 1- and 3-linked O-glycans (disaccharides) on native glycoproteins.

Key Characteristics

  • Endoglycosidase acting on O-glycans
  • Active on native glycoproteins
  • Specific for core 1 and core 3 O-glycans (disaccharides)
  • Requires pretreatment with SialEXO

product-logo-sialexo

SialEXO is a sialidase mix for complete removal of sialic acids from 2 mg native glycoprotein

Key Characteristics

  • Acts on native glycoproteins
  • Cleaves α2-3, α2-6 and α2-8 linked sialic acids
  • Hydrolyzes sialic acids within 2 h
  • Acts on both N- and O-linked glycans
  • Compatible with OpeRATOR and OglyZOR

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Patent and Disclaimer

OpeRATOR™, GlycOCATCH™, OglyZOR™ and SialEXO™

All rights reserved. Aspects of OpeRATOR™, GlycOCATCH™, OglyZOR™,  and SialEXO™ technologies are encompassed by pending patent applications in the name of Genovis AB.

The trademarks OpeRATOR™, GlycOCATCH™, OglyZOR™, and SialEXO™ are the property of Genovis AB.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

©2018 Genovis AB.