SmartEnzymes FabDELLO workflow

Above Hinge Digestion of Human IgG1

FabDELLO is a protease that digests human IgG1 at a single site above the hinge, yielding intact Fab and Fc fragments within two hours with no need for reducing conditions.

The enzyme is active on antibodies with mutated hinge regions, such as the LALA mutation, and enables middle-level LC-MS for characterization of critical quality attributes on antibodies with mutated hinges.
 

  • Robust digestion of hinge-mutated antibodies, including LALA-mutated antibodies
  • Fast - generates intact Fab and Fc fragments from human IgG1 within two hours
  • Middle-level characterization of human IgG1
Human IgG1, including antibodies with mutated hinge regions
2 hour reaction
Requires presence of calcium ions. 200 mM CaCl2 is included
…KSCDK / THTCPPCP… (above the hinge)

FabDELLO Lyophilized

Lyophilized enzyme for above hinge digestion of human IgG1

About FabDELLO™

FabDELLO digests human IgG1 at a single site above the hinge (...KSCDK / THTCPPCP…), generating intact Fab and Fc fragments.

FabDELLO is a lysine-specific protease. The single digestion site is a result of the three-dimensional structure of human IgG1 making this lysine exposed for the enzyme. Additional digestion sites at exposed lysines on the Fc may appear if the N-glycans are removed. FabDELLO is active under native conditions and requires the presence of calcium ions. Optimal activity is obtained at 37°C and pH 7-8.5.

FabDELLO is cloned from Bdellovibrio bacteriovorus and expressed in E. coli. The enzyme contains a His-tag and the molecular weight is 32 kDa.

FabDELLO™ Lyophilized

Lyophilized enzyme for above hinge digestion of human IgG1.
It is available as a lyophilized powder in 8 x 100 units or 96 x 100 units strip wells, each well digesting 100 µg human IgG1. FabDELLO is supplied with 1 vial of 20x CaCl2 solution (200 mM).
 

  • Flexible format for method development
  • Possible to combine enzymes for improved efficiency
  • Amenable to automated workflows
  • Ready to use – just add water!

Available Products

FabDELLO Lyophilized 8 x 100 unitsLyophilized enzyme for above hinge digestion of 8 x 100 𝜇g human IgG1B1-BD1-008386.00Buy / Request a Quote
FabDELLO Lyophilized 96 x 100 unitsLyophilized enzyme for above hinge digestion of 96 x 100 𝜇g human IgG1B1-BD1-0962,149.00Buy / Request a Quote

For information on how to order, visit Place an Order or contact us directly at order@genovis.com.

Unit Definition

One unit FabDELLO digests ≥ 90% of 1 µg human IgG1, when incubated in Tris buffered saline (TBS), pH 7.6, containing 10 mM CaCl2, at 37°C for 2 h.

Content and Storage

FabDELLO Lyophilized is supplied in 50 mM Tris-HCl, 150 mM NaCl, pH 7.6, with no preservatives added. It is available in 8 x 100 units or 96 x 100 units strip wells, each well digesting 100 µg human IgG1. FabDELLO is supplied with 1 vial 20x CaCl2 solution (200 mM).

FabDELLO is shipped cold and should be stored at -20°C upon arrival. Since FabDELLO has autoproteolytic activity, the enzyme should be kept cold and used the same day as reconstitution.

Instructions

Product Specifications

Safety Data Sheet

Certificate of Analysis

FAQ and Support

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