Immobilized SialEXO

365.001,295.00

Immobilized SialEXO rapidly desialylates glycoproteins without residual enzyme in the final preparation

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Description

Immobilized SialEXO contains spin columns with immobilized SialEXO on agarose beads. The immobilized enzyme acts on α2-3, α2-6 and α2-8 linked sialic acids for complete desialylation of N- and O-linked glycans on native (non-denatured) proteins and peptides within 30 min. Immobilized SialEXO is available in spin columns for desialylation of 0.5 mg of sialylated glycoproteins without any residual enzyme in the final preparation.

Detailed Information

Application

Desialylation, Exoglycosidase Arrays

Product Group

SialEXO

Source/Origin

Cloned from A. muciniphila, Produced in E. coli (His-tagged), 43 kDa, 66 kDa

Product Composition

Immobilized on agarose beads (20% EtOH)

Digestion Site

Sialic Acids

Specificity

Hydrolyzes alpha-2,(3,6,8) linked on sialic acids

Substrates

Sialic Acids

Reaction Conditions

30 minute reaction at 37°C according to protocol specifications

Digestion Buffers

20 mM Tris (pH 6.8)

Buffer Compatibility

Other buffers or pH (6.5 – 9.0) can be used but the reaction conditions need to be optimized.

Inhibitors

ZnCl2 and EDTA

Unit Definition

0.1 ml settled resin desialylates ≥ 90% sialic acids of 0.5 mg glycoprotein (fetuin)

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