SialEXO Immobilized Microspin 5 x 0.5 mg

1,040.00

Immobilized enzymes for desialylation of 5 x 0.5 mg glycoprotein in spin columns

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SKU: G1-SM6-025 Category:

Description

5 x Immobilized SialEXO microspin column desialylates 0.5 mg glycoprotein in 30 min

FAQs

Yes, SialEXO displays activity on sialic acids with α2-3, α2-6 and α2-8 linked bonds of both N- and O-glycans.

Yes, SialEXO can be incubated together with OpeRATOR and/or OglyZOR in 20 mM Tris pH 6.8.

Yes, both enzymes are active under native conditions, preferably use 20 mM Tris pH 7. If denaturing reaction conditions are required for PNGaseF, the reaction with SialEXO needs to be performed first under native conditions, then add PNGaseF and the required buffer.

Yes, you can. All three enzymes perform well in the same reaction buffer (20 mM Tris pH 6.8). There is no need to increase incubation time when combining the enzymes.

Yes, SialEXO contains a 6x His-tag.

No, SialEXO works both on glycoproteins in native conditions but can also be used on denatured proteins after buffer exchange.