Immobilized PNGase F Denaturing


Immobilized PNGase F for removal of N-glycans from glycoproteins under denaturing conditions.



One microspin column of Immobilized PNGase F contains sufficient material for removal of N-glycans from 0.2 mg glycoprotein. PNGase F is an amidase that digests the bond between the innermost GlcNAc of N-glycans and asparagine residues on the glycoprotein backbone. It is active on high-mannose, hybrid and complex-type N-glycans. RapiGest™ SF is a surfactant used to enhance enzymatic digestion of proteins. It helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity.

Detailed Information

Product Group


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Product Composition

Digestion Site



Reaction Conditions

Digestion Buffers

Buffer Compatibility


Unit Definition



The columns can be used for deglycosylation at native or denaturing conditions. Reducing conditions can be used with the denaturing protocol but the reaction parameters need to be optimized for the specific substrate.

Yes, it is possible to perform the incubation at 37 °C if the incubation time is prolonged. Treat the protein sample mix containing 0.5% RapiGest at 90 °C, 5 min prior to the incubation with Immobilized PNGase F.

Immobilized PNGAse F hydrolyzes the amide bond between the polypeptide asparagine and the innermost GlcNAc of all mammalian asparagine-linked complex, hybrid, or high mannose oligosaccharides. It does not remove N-glycans with α1-3 core fucosylation.

No, it is not recommended. We can only guarantee optimal performance for one-time use.

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The recommended buffer for best performance of the column is TBS buffer at pH 8.6. Other buffers with neutral pH containing 0.15 M NaCl can also be used but optimization may then be required.

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